FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22149717
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT8451993.8158455929707817TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1615050.053.584879
AGAGCAC1630700.053.0589568
AAGAGCA1738200.049.8486567
TCGGAAG1742700.049.668153
CGGAAGA1743700.049.6495554
ATCGGAA1760100.049.169712
GATCGGA1786800.048.3555761
GAAGAGC1833700.047.3146826
TCGTATG941450.045.80132744-45
CGTATGC944150.045.51685346-47
TATGCCG945900.045.4448448-49
CTCGTAT947600.045.16823244-45
TATCTCG949850.044.84774440-41
TCTCGTA961500.044.45310242-43
ATGCCGT983200.043.83189848-49
ATCTCGT976550.043.6731742-43
GTATGCC990800.043.52958346-47
GCTATCT981000.043.3842438-39
GGAAGAG2011950.043.2101555
GCCGTCT997050.043.20810350-51
Produced by FastQC (version 0.11.5)