FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28571400
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG542216718.977603477603477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA99750.041.0593534
CGAGCAA101650.040.4181568
TTGTACG101000.040.4030383
TACGAGC100250.040.232296
CCGTCGA27800.039.9051487
GTACGAG102950.039.6154825
CCGTGTT100350.039.087761
GTTCCTC99100.038.854915
GTGTTCC99050.038.615413
GATGTCA154350.037.747769
GGTTGCC140700.037.3441961
AGTCACC83150.037.1630442
TGCCAGA146050.036.8900764
TTGCCAG144700.036.789283
TCCGATT26800.036.661286
AGCAGTG246700.035.9298672
CTGCGAA29450.035.84374212
TCTGCGA29750.035.33893611
AGATGTC165900.035.300758
TTCCGAT28050.035.1045
Produced by FastQC (version 0.11.5)