FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_Yen001-cDNA_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_Yen001-cDNA_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91298426
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4190890.45903201003706245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3415900.038.5078661
AGCAGTG3578700.036.5884632
AGTGGTA4437350.029.489385
CAGTGGT4467700.029.2773884
GCAGTGG4545000.028.931583
TCAACGC4531100.028.5284112
CAACGCA4539650.028.51186613
ATCAACG4570350.028.37666111
GTGGTAT4605750.028.3570426
CGCAGAG4644250.027.9843916
GAGTACA4631850.027.85517520
AACGCAG4669950.027.77778414
ACGCAGA4665550.027.72826615
GTACATG4638650.027.72173922
GGTATCA4716850.027.633068
TACATGG4641050.027.62598223
TGGTATC4728850.027.5620147
GCAGAGT4732100.027.37922717
AGTACAT4709150.027.3046721
TATCAAC4789850.027.07133110
Produced by FastQC (version 0.11.5)