FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_Yen001-cDNA_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_Yen001-cDNA_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92475590
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4158200.44965379512582726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3498300.038.558841
AGCAGTG3659900.036.7054672
AGTGGTA4543550.029.5553595
CAGTGGT4578950.029.3450434
GCAGTGG4643000.029.0318983
TCAACGC4653900.028.5011912
CAACGCA4658700.028.49940113
GTGGTAT4723200.028.3966876
ATCAACG4692500.028.34931811
CGCAGAG4767350.027.97104616
GAGTACA4753900.027.84205220
AACGCAG4789300.027.82333614
ACGCAGA4780500.027.78691915
GTACATG4761850.027.73736422
TACATGG4765600.027.62756523
TGGTATC4850700.027.5983927
GGTATCA4852350.027.588088
GCAGAGT4853400.027.40102217
AGTACAT4835950.027.29430221
TATCAAC4909450.027.1212410
Produced by FastQC (version 0.11.5)