FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_Yen001-cDNA_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_Yen001-cDNA_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139184506
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6012810.43200282652150945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5272450.038.555311
AGCAGTG5528000.036.633542
AGTGGTA6840700.029.5871055
CAGTGGT6889100.029.3732074
GCAGTGG6975650.029.1025493
TCAACGC7009000.028.5522212
CAACGCA7018850.028.54574813
GTGGTAT7106450.028.435926
ATCAACG7062550.028.4233611
CGCAGAG7161900.028.05389216
GAGTACA7157200.027.89414420
AACGCAG7215500.027.84485214
ACGCAGA7201400.027.79390715
GTACATG7169350.027.75587722
TACATGG7171250.027.68088723
GGTATCA7299100.027.6556728
TGGTATC7301150.027.6383957
GCAGAGT7295750.027.47422617
AGTACAT7270450.027.37204421
TATCAAC7404850.027.13018210
Produced by FastQC (version 0.11.5)