FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_Yen001-cDNA_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_Yen001-cDNA_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101926301
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4346300.4264159453799859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3837300.038.484481
AGCAGTG4010350.036.6627242
AGTGGTA4976050.029.5464535
CAGTGGT5003550.029.3896374
GCAGTGG5074550.029.066463
TCAACGC5076800.028.60270512
CAACGCA5086700.028.58243213
ATCAACG5115500.028.47030611
GTGGTAT5159150.028.452576
CGCAGAG5197150.028.08707416
GAGTACA5184100.027.98027220
AACGCAG5231550.027.86593814
GTACATG5192700.027.83853122
ACGCAGA5220650.027.8307415
TACATGG5196500.027.7572123
GGTATCA5291450.027.7088018
TGGTATC5290300.027.704197
GCAGAGT5294750.027.50774817
AGTACAT5270550.027.45300921
TATCAAC5367250.027.16881210
Produced by FastQC (version 0.11.5)