FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n02_Yen001-ADT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n02_Yen001-ADT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26845729
Sequences flagged as poor quality0
Sequence length49
%GC17

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550843.9301745167732265No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10514333.9165745880843836No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10476333.9024196362855332No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9349233.482576315957No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7853872.9255566127483443No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7818932.912541507068033No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810702.5369771109586927No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6627962.4689066927554846No Hit
CAGTAGATGGAGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6511472.425514315517377No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5043131.878559528035167No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4802051.7887575338334079No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4515001.6818317729423553No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4445761.6560399607699234No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3509731.3073699730783992No Hit
CAGTAGATGGAGCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3466341.2912072531165013No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3332391.2413110480255536No Hit
AATTAGCCGTCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3154081.1748907992031061No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3089321.1507677813480126No Hit
GCAGAAATCTCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2658240.9901910281520013No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2273050.8467082417467597No Hit
AATTAGCCGTCGAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2204370.8211250288639954No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2161030.8049809338386751No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840160.6854572658466455No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1837030.6842913448168981No Hit
AGACTAATAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1679350.6255557448262999No Hit
AATTAGCCGTCGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1654690.6163699261063091No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1633530.6084878529467388No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540750.5739274206336509No Hit
CTCGTTTCCGTATCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410830.5255323854308446No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390530.517970661180406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250690.4658804385606366No Hit
TGGTAACGACAGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1163580.43343207405542983No Hit
AGACTAATAGCTGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA984650.36678087601942194No Hit
CAGCCATTCATTAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA926500.34512007477986534No Hit
AGACTAATAGCTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA882080.3285736811244723No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT786810.2930857269698282No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT769410.28660424904088094No Hit
CAGCCATTCATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA735460.2739579171048028No Hit
TGGTAACGACAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA716530.266906516116586No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG710070.26450017431078143No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT707250.2634497278878141No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT698170.260067439405352No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT693720.25840982004996027No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685030.2551728060728021No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG645500.24044793121468225No Hit
TGGTAACGACAGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA636880.23723699214873248No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG619820.23088216378851176No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG573220.2135237228983426No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT557850.20779841739443916No Hit
CAGCCATTCATTAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553720.20625999763314307No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT545610.20323903292028314No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG543130.20231523606604238No Hit
CTCGTTTCCGTATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512140.19077150037534835No Hit
GAGATGTCTGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510360.19010845263319168No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG505220.18819380915303138No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT500180.18631641554602596No Hit
CTCGTTTCCGTATCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA498590.18572414256286354No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC493890.1839733985245847No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC492740.1835450249833037No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC484050.18030801100614552No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC483640.18015528652621055No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG453320.16886112498565414No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG448130.1669278565689164No Hit
GAGTGTCTAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA428050.15944808203941863No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT424180.15800651194832518No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC420590.15666924150206538No Hit
GTGTGTTGTCCTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA413360.1539760756729683No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG411570.15330930294349615No Hit
CAGTAGATGGAGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT404090.15052301243151192No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG387410.1443097335892797No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC380520.1417432173289092No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG380230.14163519269676006No Hit
GTCCCTGCAACTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA364410.13574226276365972No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT348110.129670533439416No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC347790.12955133384532042No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT343390.12791233942650615No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT332050.12368820381074397No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG328010.12218330893528724No Hit
GAGTGTCTAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327490.12198960959488192No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT324400.12083858851439647No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC311310.11596258011842406No Hit
GAGTGTCTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310850.11579123070191166No Hit
CTTTGTACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302480.11267341631884908No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302220.11257656664864643No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT296130.11030804937351486No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG289370.1077899579482457No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT286650.10677676139843324No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT279190.10399792086107999No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG277790.10347642263691181No Hit
CAGTAGATGGAGCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT275740.10271280023723699No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG273310.10180762831957367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1450.043.0279661
CTTTTGT2450.043.0279661
CCCTGAT800.043.027961
TCACGCC50250.043.027961
CTTCGTA600.043.027961
CTCGTGT401.0140866E-843.027961
GAGTCTC1500.043.027961
ACGTTGC208.773443E-443.027961
GAGTCGC4600.043.027961
GCTATTG255.072001E-543.027961
CCGTGCT650.043.027961
GACCCTG255.072001E-543.027961
CCGTCTT6350.043.027961
CCGTCAT850.043.027961
CTCGGGT208.773443E-443.027961
ACGTGTA255.072001E-543.027961
CTTTGGC208.773443E-443.027961
TCACTCC1150.043.027961
CCGACTT600.043.027961
GAGTTTC850.043.027961
Produced by FastQC (version 0.11.5)