FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28571400
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG11018963.8566398566398568No Hit
GGGGCGGGGGGGGGGGGGGGGGGGGG363140.1270991270991271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC205900.019.5692254
GGAGCGA213150.018.8990146
CGATAGC217500.018.62463810
GAGCGAT216950.018.551957
GTTCGGG239050.018.380321
GACTAAC56500.018.0782661
GATAGCC77950.017.62606811
GCGATAG232250.017.4976839
ACGAGGA50250.017.3404981
GCGCGAT25350.017.324711
CTCGAAA44400.017.0791281
GGGAGCG231750.017.0671485
ACATGGT85300.017.0293081
GGGAATG32700.016.9488981
ATGGGAG36600.016.8648911
TTCGGGA238600.016.833612
AGGCGAA20350.016.80531110
GACCTGG34650.016.8034781
GGGACCT28550.016.7494771
GCGGGGG48700.016.6953034
Produced by FastQC (version 0.11.5)