FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_Yen001-cDNA_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_Yen001-cDNA_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91298426
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC365400.017.9764691
TGACGGC268600.017.937111
TGAGCCG229200.017.8473831
GATGAAA320500.017.7730311
CTGCGGA214000.017.572381
CAATGGA277100.017.552538
AGGCGAA168250.017.46168110
TTGAACG240550.017.4377961
CTCGAAA299800.017.2516631
GGGAATG240700.017.2149621
ACGAGGA358750.017.208211
TTGGAAC290600.017.1093241
GACCTGG265350.017.0898671
ACGAGCC282450.017.0788121
CGGGAGC169200.017.0753864
GCGCGAT177400.017.0586171
CATGGCG227900.017.0141981
GAATGAA246750.017.0077511
CCGGGAT189350.016.927751
GGGACCT195600.016.8727361
Produced by FastQC (version 0.11.5)