FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_Yen001-cDNA_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_Yen001-cDNA_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92475590
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC363800.018.0797651
TGACGGC264050.018.0638281
TGAGCCG238800.017.8080561
GATGAAA322600.017.7560631
CTGCGGA210350.017.5343761
AGGCGAA179850.017.48089410
CAATGGA279300.017.4717228
CTCGAAA303150.017.3872281
TTGAACG240400.017.3441471
ACGAGGA367400.017.3226451
GACCTGG269450.017.2117481
GGGAATG243650.017.1579631
GAATGAA246250.017.127111
ACGAGCC295450.017.0209831
TTGGAAC292050.016.9416851
GGGACCT197250.016.9339831
AGGGATG262100.016.9272461
CATGGCG229200.016.9172151
CGGGAGC171450.016.8860934
GGATTAC307600.016.8722651
Produced by FastQC (version 0.11.5)