FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_Yen001-cDNA_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_Yen001-cDNA_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139184506
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC562200.018.023721
TGACGGC387650.017.9974441
GATGAAA489800.017.7569981
TGAGCCG355200.017.7011281
AGGCGAA265600.017.55236410
CTGCGGA324000.017.5098841
CAATGGA417750.017.473938
TTGAACG377200.017.4564081
GGGAATG375100.017.3807831
ACGAGGA553650.017.3606661
CTCGAAA440700.017.1997811
CGGGAGC263950.017.1404154
GAATGAA372750.017.1173111
ACGAGCC432900.017.0521281
GGGACCT302950.016.9697841
GCGCGAT272050.016.9409661
GACCTGG407150.016.931541
TTGGAAC441450.016.8963551
GCTGCAG438450.016.8773481
GGATTAC443000.016.8620781
Produced by FastQC (version 0.11.5)