FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_Yen001-cDNA_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_Yen001-cDNA_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101926301
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC289200.017.8910581
TGAGCCG265300.017.8813421
GACTAAC412800.017.8415221
GATGAAA365800.017.801081
CTGCGGA237100.017.6157341
AGGCGAA197150.017.61053310
CAATGGA305800.017.582748
TTGAACG275150.017.3648341
ACGAGGA398200.017.3048461
CGGGAGC186000.017.2967514
GAATGAA275300.017.2245521
GGGAATG274500.017.2200851
ACGAGCC314550.017.1743321
CTCGAAA327350.017.1598381
GACCTGG297950.017.0836051
TTGGAAC323700.016.991741
CATGGCG246450.016.975831
AGGGATG292500.016.8957271
GCGCGAT200700.016.8777831
GGGACCT220700.016.821491
Produced by FastQC (version 0.11.5)