FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n01_Yen001-HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n01_Yen001-HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17636832
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGG7275400.019.966111
TTCGGGA7317150.019.9010812
CGGGAGC7322850.019.853664
TCGGGAG7347550.019.8512443
GGGAGCG7341950.019.8193685
GGAGCGA7355200.019.8041156
GAGCGAT7367100.019.785497
AGCGATA7392600.019.7617768
GATAGCC2520550.019.75715311
CGATAGC7377750.019.73417710
GCGATAG7402000.019.7170479
ATAGCCG590500.019.66047112
GATAGCA2623550.019.65840711
ATAGCCA620450.019.6397512
ATAGCCT758100.019.62850412
GATAGCT1711150.019.60820411
GATGAAA119000.019.5295371
ATAGCCC574300.019.52903612
ATAGCAG587950.019.52804412
ATAGCTG339000.019.468212