FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-cDNA_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-cDNA_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182596852
Sequences flagged as poor quality0
Sequence length26-49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG4719900.039.90513223
ACATGGG4789300.038.60508724
GTACATG4894350.038.57108322
AGTACAT5015350.037.66715221
CATGGGG1841900.036.31350325
CATGGGA2500700.035.85939425
ATGGGAT989050.032.29630326
CATGGGC714800.029.8262525
ATGGGGA980450.028.58906726
ATGGGGC529400.026.40210326
AGCAGTG3672700.026.3603362
AAGCAGT3689750.026.3311671
ATGGGAA966800.025.64313726
GACGCGT255950.025.1991211
TGACGGC295000.024.977631
TACGGAT298100.024.5963041
ATGGGGT521200.024.10370426
AGCGATA281000.023.9374378
ACGCCGA324300.023.6393031
GCGCGAT198700.023.632911
Produced by FastQC (version 0.11.5)