FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-cDNA_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-cDNA_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184951180
Sequences flagged as poor quality0
Sequence length26-49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG4852600.039.95003523
ACATGGG4922650.038.64256324
GTACATG5030250.038.62420322
AGTACAT5149850.037.73280321
CATGGGG1883550.036.8186925
CATGGGA2554750.035.7853925
ATGGGAT1009600.032.01382426
CATGGGC743150.029.47288125
ATGGGGA995800.029.1389726
ATGGGGC533000.026.53741626
AAGCAGT3777750.026.4974421
AGCAGTG3779850.026.3851182
ATGGGAA994950.026.23512626
GACGCGT254350.024.9855541
ATGGGGT536900.024.91886926
TGACGGC306150.024.7727451
TACGGAT294800.024.5933271
AGCGATA279250.024.211678
GCGCGAT201450.024.045241
TCGCGAG309650.023.8769841
Produced by FastQC (version 0.11.5)