FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-cDNA_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-cDNA_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278369012
Sequences flagged as poor quality0
Sequence length26-49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG7321250.039.8549823
GTACATG7580000.038.5958822
ACATGGG7431600.038.51640724
AGTACAT7760650.037.71978821
CATGGGG2857800.036.347625
CATGGGA3859900.035.8833725
ATGGGAT1524400.032.1114926
CATGGGC1100150.029.55811725
ATGGGGA1504600.028.90776826
AAGCAGT5675050.026.4991021
AGCAGTG5679050.026.3740522
ATGGGGC813050.026.195726
ATGGGAA1486500.026.04281426
GACGCGT385550.024.9965421
TGACGGC446550.024.8954541
ATGGGGT803500.024.79767426
TACGGAT450200.024.5011061
AGCGATA426250.024.1787158
TCGCGAG456250.023.7686731
GACTAAC673300.023.6798631
Produced by FastQC (version 0.11.5)