FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-cDNA_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-cDNA_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203852602
Sequences flagged as poor quality0
Sequence length26-49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG5312500.040.10338623
GTACATG5505850.038.78552222
ACATGGG5386050.038.74031424
AGTACAT5651500.037.80216221
CATGGGG2064200.036.70520425
CATGGGA2795000.035.86976225
ATGGGAT1097550.032.18682526
CATGGGC812450.030.13065125
ATGGGGA1090900.029.330726
AAGCAGT4143950.026.463881
AGCAGTG4129700.026.463492
ATGGGAA1068250.026.28408826
ATGGGGC577950.026.18512326
TGACGGC327900.024.9555361
ATGGGGT587100.024.52680826
GACGCGT276400.024.4288371
TACGGAT329750.024.41891
AGCGATA307750.023.939658
ACGCCGA366350.023.846081
TGGGATA551750.023.82741527
Produced by FastQC (version 0.11.5)