FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35273664
Sequences flagged as poor quality0
Sequence length26-49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC29050.042.7221837
AGAGCGT29800.042.39000336
TCGTGTA25700.041.057342
GTCGTGT28250.039.91641
AAGAGCG32700.039.59770635
GCGTCGT32000.039.3733439
CGTGTAG26800.037.84541743
CGTCGTG33400.037.43940440
CGGAAGA38900.032.8849232
TAGATCG18050.031.94073727
GTTCGGG7255450.031.4448641
GGTTGCC8341500.031.4250761
GTTGCCA8370950.031.4193082
TTGCCAG8453150.031.4020233
CCAGATG8485700.031.3607966
GCCAGAT8563050.031.3600525
TTCGGGA7299000.031.340442
TGCCAGA8492550.031.340354
CAGATGT8495500.031.3369067
AGATGTC8510750.031.3364418
Produced by FastQC (version 0.11.5)