FastQCFastQC Report
Wed 30 Jan 2019
HGYGTBGX9_n001-ADT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGTBGX9_n001-ADT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53691458
Sequences flagged as poor quality0
Sequence length26-49
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG4464600.031.3447575
TTTGTAC4474100.031.313122
TTGTACG4481400.031.3066773
GTGTTCC4370750.031.3039633
CTTTGTA4488950.031.301431
CCGTGTT4417200.031.2904531
TACGAGC4469200.031.280546
TAGATGG2547550.031.278564
ACGAGCA4484050.031.2711477
AGATGGA2555650.031.2675515
TGTTCCT4376700.031.2632034
TGTACGA4495650.031.256844
GCAGAAA1659650.031.2529091
CGTGTTC4354450.031.237382
GATGGAG2547450.031.2347726
TCACCAA3699500.031.2259564
CGAGCAA4492750.031.2221858
GTCACCA3692000.031.2070563
AGTCACC3697850.031.1515372
CACCAAT3708450.031.1484685
Produced by FastQC (version 0.11.5)