FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380289208
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37529330.9868628720066124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA5501500.014.0870061
TATACTG3014600.013.5554155
GTATTAG2398550.013.3821661
GTATAGG1456750.013.0552481
GTATAGA2343000.012.7659511
GTGTATA3732850.012.3956061
GTATTAT3848900.011.6869281
TCTATAC2225400.011.6248913
CTATACT2264600.011.5555674
TATACAG3117350.011.3886945
CCTATAC1402000.011.1767523
GTCCTAG1553200.011.1441241
TACACTG2889250.010.9160865
CTAATAC2373550.010.7070123
TATATAC5467650.010.6686673
ACTGATC2519500.010.5114168
GTATAAG2707100.010.42561151
GTGTAAG2370550.010.4148571
TAATACT3315500.010.3511684
GTACTAG1097200.010.3366881
Produced by FastQC (version 0.11.5)