FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_negctrl1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_negctrl1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCAACATGCTCCACCTAACGAATATGTGAAGCAGAAAATATTCCAAC10.6944444444444444No Hit
GATAGGTGACTAATTGAATGGTATTCAGATGTCTTTTGTCTGGTGGTTCA10.6944444444444444No Hit
GCTTAAACACGGTATCTTTCTTTGTATTAAGCCCAGGTTTCACAAAAAGA10.6944444444444444No Hit
TCATATTAGAACCACTTAATGGACAACAATCTCCTCTGATCTCAGGAAAA10.6944444444444444No Hit
GTTCATGAGTTGGACCATCTTCTCCGACTGCGATTGAATCGTGGGGGAAG10.6944444444444444No Hit
GGAAGTATTAAGGAAGGGATCAGGACAAGAAACCATGTATTCGAGTATCA10.6944444444444444No Hit
GACAAGGCGGGCGGCACGCTGTACCGATGGATATAGTAGGAATTGTTCAA10.6944444444444444No Hit
GCCAAGGCCGCACCGCGCCCTCACCCACCAGGAGTCCCGGGCGGGTGCAC10.6944444444444444No Hit
TAAAATGACTCAGCTAACCAGCATAATATAAGCCCGTATTTAAACTTTGT10.6944444444444444No Hit
CTGAAGGCAGAAAAGGAAGTAGAAGATAAGGTGCACAAATCACCAAGCTG10.6944444444444444No Hit
TTCACACACTGTAATGAAACAGATTCATAGTTTCAATGTCATTGTTTAGT10.6944444444444444No Hit
CATAAAATGTCTAGTAGCCATAAGAAAAAAACTTTCGTGGACTTAGTTGG10.6944444444444444No Hit
GAATAAAATGCCTAGGAATCCAACTTACAAGGGAAATTATGGACCTCGTA10.6944444444444444No Hit
AGATAGCTGAATAATAAAGTACACAGATTAAGGCTACCAGCCAGAAGCCC10.6944444444444444No Hit
CGCCTGCCCCGCGCGCACCTGCCCGGCGTGCCCCGCGCATGGGTCCCAAG10.6944444444444444No Hit
CCTCACCTACAAGAACCTCACATACCTTAATAAAACATAAAATAAACACA10.6944444444444444No Hit
GGTTAGGACTTCAGCATATGAATTTCTGGGCAAGGGATGAAGAGGGTTGG10.6944444444444444No Hit
AGTTGGTGCCAACCTAATAAAAAACCGACTTTCACGCACCAGAACGACCA10.6944444444444444No Hit
TATCACACCACTTCCAAACGGCAGCGGGCACCGGGCCAGACTTCTTTAAA10.6944444444444444No Hit
TGCCTGTATTTTACAATGTATTTGTACGGGGTACAAATACTGGCTGATCA10.6944444444444444No Hit
CTTGTGAAACAGTGTCAACAGGATTGGTACCAATTACTTGAATGTCTGGT10.6944444444444444No Hit
AGTTTTCAAAGCCTGTGCGCCCGTGCGTCGCTCCCGAATGTGGACCGCCT10.6944444444444444No Hit
AGGTATACGTGAGGCAAGGGCAGTGAACTTGACTCTCTAGGAACTCCTTC10.6944444444444444No Hit
TGCCAAATGTATTTTGCAATTCTTGTAGGAAATGGGGCAATTCCAGAAGT10.6944444444444444No Hit
TTATTGTACATATCTACAATTTTCTTTAATATCCTATTATGTTACAACTT10.6944444444444444No Hit
CATTCCCGAAGGGATATGTTTGCTGGGCTCTCTGACTGGAACAGTCTCTC10.6944444444444444No Hit
GTAATAGATAAGTTTATGGCTATAAACGATATGGAAAATGAAGCAAAGAA10.6944444444444444No Hit
TGAAAGGAGAGAGCAGCGGGCCAGCTTCATAGAACTTAGCTCCCGCCTCT10.6944444444444444No Hit
CTACAGTATCGATCTTGACTACATATCAGACGAAGAACTAATTCCTCTCT10.6944444444444444No Hit
GCGCGTGCTACAGGACGGCTTCATCTGATTCGAGTCGAGAATATTCCAGG10.6944444444444444No Hit
GGTCATGTTTTCCCATTGGTAGATAAAGAAGGTGGCTTTCTCGTACGTCA10.6944444444444444No Hit
GTGGTAAATGCATGAAATACCATATAATAAGGATGCTGAGTAAGTAGAAT10.6944444444444444No Hit
ACCTCCACTCTGCAATCATTGGATGCAGAATCCTGGGAAGGAGACATGAC10.6944444444444444No Hit
AGGCGGGGAGAAGCTAGGTCAACGTAGAGAGCGAATCTGTGTGGAGCCAG10.6944444444444444No Hit
GAATGCCGGCATTCCTGTATAGACCCCTGACTCTGACGACATCCTGCCGA10.6944444444444444No Hit
CCTCATATGAAACCCATCCTGGCCTGACACGGAGCATATTCGCGGACGGG10.6944444444444444No Hit
GCCTTCAACGTGCTACAGATATTCCTTTTTCAAACCATCAATGGTAGATT10.6944444444444444No Hit
GAATATAACGAATTTGACAAATAACTATAACTCAGAAATTCAACCCGCGG10.6944444444444444No Hit
AGGTTATTATTTATGAAAAAGGAGAATCATCTATGCTATATATTTGCGCT10.6944444444444444No Hit
GCAAAAAGAGAGCTTCTGCAGGGAAAATCTTCCTAATAAAACCATCAGAT10.6944444444444444No Hit
GATTGAACAGAACTCTAATTAATACACATCTATGGCAACAGGCATTGATG10.6944444444444444No Hit
TTCATTAGCGAGAGGGATTATGACAAACATTTTTTACTAAAAAAATCTTA10.6944444444444444No Hit
GACCATAACAATGCGATAGGAATAGCGAGCGTAACCTTAATCTTTAAAGT10.6944444444444444No Hit
GGGGACATCAGAGGCGAGGAGCACCAAGCAGTTCACACAACACAGATCAC10.6944444444444444No Hit
CATGTGTAGCATCTAAAAATGTTGAATTCATAGAAACAGAAAATAGAATG10.6944444444444444No Hit
TTGTACATGACCATAATGCGTCCTCTAGCGCAGGTCCGCCCCCTGTTGTC10.6944444444444444No Hit
ACCGAATTCAATCCCTACAGAGTTACTCAGTGCAGGCAACCAGTAGGCCA10.6944444444444444No Hit
CCTCAACCTACACTCCTATACATACCTTCCCGATCATAACATACTACACT10.6944444444444444No Hit
GGCAGAAGAATCACTTGACCCTGGGATGCGGAGGTTGAAGTGCGCTGAGA10.6944444444444444No Hit
ATGGAGGCGATGTGACGCAGGCCATTTATTGTTTTCGAATGGAAGAGAGT10.6944444444444444No Hit
GGTGTGGCGACACCCGGCTCAGATCAGGATGCTCGTCCGCCTTTATCACC10.6944444444444444No Hit
AGCCCGGGCGCTCCAGGCGCTGGCTGCAGTGCCTGTGGCGGGGGCCGGGC10.6944444444444444No Hit
CCATGGTCCGCAAGATGGCACGCCCTTTGAAATGACGCCATCCCGCAGGG10.6944444444444444No Hit
GAATATCCCAGTGAAGAAGTGAAAACACTAGTTGTGAAGAAANNCTCTAT10.6944444444444444No Hit
CTAGAGTATAGTAATTTAACCTGCTTTACTGCTTTTCTAACGATATTTCA10.6944444444444444No Hit
ACTTGTTAGTGGCTATAAGAAAACAAGGGTCTTCTACTCGTTTTTCTTCT10.6944444444444444No Hit
GCCAATACCCTACGGCAGATGACACTGCTAAGCAGCGCTTCATGGACGAT10.6944444444444444No Hit
ATGTCCCTTCAGGTCTGTGGTTATCGGACGTGCCGGCCGTCCGGGCGGGA10.6944444444444444No Hit
ACTCAGAATGTATTTATATCATAGTAACTGCTATTTTGTGGTAAGTTGGA10.6944444444444444No Hit
TGCCTGTGTTGTATGTATTAGTAGTACAATATTCGTGGTTTTCACCGCCT10.6944444444444444No Hit
GGCCTACATTGTGACAGAGAAGAGAGGGTTAACATCAGTCGGGGCCAGAC10.6944444444444444No Hit
ATAATTACCTGCCATTAATTCTGGATGTTGCCCGTCAAGCCAGCGCCACC10.6944444444444444No Hit
ACGACAATAACTAGCCATTGGAGGCATTTAAGCAGGCACATGATATAATC10.6944444444444444No Hit
GCCGCATGCCGCTCCGCCTACCAGTTCGTGACCCTCACTCGCGCCGAACT10.6944444444444444No Hit
TCACCCTACAGATTTGCAAACCAAAAATATTGCTAATAACACATTTATAT10.6944444444444444No Hit
GCCTTGTTACCAGGAGCAAGTGGCAGGGAGGATGGTCCGGCATGACAAGG10.6944444444444444No Hit
TAGTAGGGGCTTTCTGCTTGATGCGGGCAAGGGGAGGGGACTTGGTGTAA10.6944444444444444No Hit
GTGTAAAACATGCCCTTTTGCAAAGCAAAAGATGCCCGTTAACACGCCTG10.6944444444444444No Hit
TGTGTTGGCTGCATAAATGGCTTCTTTTTAGAAGGCTCTGTTCATATCCT10.6944444444444444No Hit
GACGAACTTTGGAGTACAGACTTTCTTGTCACCCTTCCTTAAGTTTGCTC10.6944444444444444No Hit
TGACATTACTTTCAGAATCCTAAGGTGAGGTGATTGTGAGTGATGATAAA10.6944444444444444No Hit
TACTCACCCTGTTTTCATCCTTTGCCTCAGAACTTTACTAAAAAGGTTTT10.6944444444444444No Hit
ATTCTGTATCTCTGTATATGCATACAGAACTAAGAGTAGAAGAGGATAAA10.6944444444444444No Hit
CTCCCAAAGGTTCTGAAGGATAACGTGCAGCCCCCTATCCAACTGTCGGC10.6944444444444444No Hit
ATGATGAACAGGCAATTATAGGTAAAATTTACTTGTACATGGGACAAAAA10.6944444444444444No Hit
GGCCTGAATGCCTGCGAAGACACACATGGCATGTACTCTGTGTTATTGGT10.6944444444444444No Hit
TTACAAGAGTCACTAAATTGGCTCAAGTTGCTACATGTAGGCGCCGGATT10.6944444444444444No Hit
GGGCTCGGCTGAGGAGTGCGGCAGCCTGGGGAAGTCCCTGGGCTTGAGCA10.6944444444444444No Hit
AATATAAGATATAGCCCAGATTTGATAATAACAGAAAGGGGTATCATATG10.6944444444444444No Hit
TATGTGTTTTGTTGGTATAGACCCTGCCTTTACATTAAGGGATCAGCCTT10.6944444444444444No Hit
GGCCAGGAATGGAGGCTCATGACTGTAATCCCAGCACTTTCCGACGCTGA10.6944444444444444No Hit
TGACAGGACTCCTTCTTTGATAACAGTGACTTACAGCTTACTCTGAGCAT10.6944444444444444No Hit
GTTCTGGGTTGACATGCATGAGCCACTGTTCTTGGCCTACTGGAAAAATT10.6944444444444444No Hit
TCTATAAACTCATCACCCAGGTGACATAAATATTATCTATGATCTGACCA10.6944444444444444No Hit
CTTTCAAGAGATTGAAAAAATGAAAACCACATGGCTGTACGTGTCGGTAT10.6944444444444444No Hit
CAAGATACCAACCCGAGCGAGGCCGACGGAAACTCTAGCATTGTAAACCA10.6944444444444444No Hit
GTCTACCTGCCGGCCTCGGCAGGTGGCGACCGCCTCGACGAGAAATTTGA10.6944444444444444No Hit
GAGGAATTGTGATATGGTCTCCGAATAGAATATATTTCAGTAGTGCAAAT10.6944444444444444No Hit
TATCCCACCTTTCCTCTTCCGCTTTCCCGATATGCAGTTTGGTTTGGCTC10.6944444444444444No Hit
TTCTTTCTCTATCTCATTTTCATGGCTGAATGGATTAAGCCAAGATGAAT10.6944444444444444No Hit
GTTCAATTGTTGGTTAGGCAAAACCAACAATATTCAGACGAGTCACACAC10.6944444444444444No Hit
GGAAAAAAAAAATTAAACCTGAATTTAATCAAGCCTTTATCTCGTTCAAT10.6944444444444444No Hit
GATACGCACTGCTTGTTTCACTAGTGCCATATCATCGAATAAATTGAACG10.6944444444444444No Hit
GTCCTGACGACCTGTGGCCAAGGTGGATGGTGTACATCTTAGTTTTATAC10.6944444444444444No Hit
GCGGCACTCATGTTGCTAGCATATTACAAACCCATTAAAAAGAGGTAGCG10.6944444444444444No Hit
TAAACATATTTATAAATAACTTGTCAAATGGGACATTAGTGATTCAAATT10.6944444444444444No Hit
GAATGGACTTACCCCGAGTGGCATGGAATGGAAGGGCCCAGACTCACCCC10.6944444444444444No Hit
GTTGTAGAACAAGGCATGGTCATCGAAGCTGAGGTTGCTCGCCGCACACT10.6944444444444444No Hit
ATGTCGCACCATTATTTATACACCTCGCGTGCCAATAAAGGGCAAATGCT10.6944444444444444No Hit
GTCGTGCAGCTTCAAATAAATTCAAAACCAAATTAAAAACTGCGCATCCA10.6944444444444444No Hit
ACAATGAAATTTAACTATAGGATTTTTTGAGGCCTCGTATTAAATTGCAT10.6944444444444444No Hit
ATATTTACATAAATAGTTTATACCTTCTAGAGATACATAAGGAAATGATA10.6944444444444444No Hit
CCTACAGTAAAACCACGTATGATTTTAGCATCATAACCGCCTTGGATGCT10.6944444444444444No Hit
GTTTTGCTCTTTCTCCCAGGCTTGAGTGCATTGGTTAACTCTCGACTCCC10.6944444444444444No Hit
GTATGAGCTGCCGCGCCTGGCCTAAAATACTTAAGTTTTACATCAGAAAA10.6944444444444444No Hit
GTACAAATATCTATCAAGAGACTTAGACGAAGAACCAAAATAAACTGATG10.6944444444444444No Hit
GTTAGTTTCAGCAAACCCCAATGAAACATGAATACCTATATAACAAACAT10.6944444444444444No Hit
CTACCGAGTTTAGGAGAATGAGGGAAGTGTGGTACTAGTTATACTAGAAA10.6944444444444444No Hit
ACATAAAATAAAAAAAGATAATGCAAGATAAATTAATAACAATCAATATA10.6944444444444444No Hit
GGGTGGAGCTTGCCTTGGTTGTGTTTCCCTTGTGGGTTGGTTTTGGAGTG10.6944444444444444No Hit
ATTAGGATCTGTGTTCGCGAAGGAAGGAAGCGAGACCGTTGGGGTGCGAG10.6944444444444444No Hit
ATACAGATCCCCCCGCTCGACTGTTGGGGCATTTCCACAGGAAGAACAAC10.6944444444444444No Hit
TTTACCTAACATAACTCAAATGAATAGGTCAGTTCAGGTGAATTCCCAAA10.6944444444444444No Hit
CATTAGTTCCTTAAAATTAATGGCAAACATGTCATTCCTACATAATTCAG10.6944444444444444No Hit
CATCTGGGCTGTGAACTAAAGTGACCAATGCAAACTATGGGGGGTTGCAC10.6944444444444444No Hit
GCTTAAAGTACTATCCATCCCAGGGATTTGTGCTTGGAGAGTAATGTCGC10.6944444444444444No Hit
CGAGGAGCTTGTAGGATACCAGAACGAGGCGTACAACCGGCTCAACGTGG10.6944444444444444No Hit
CTGTAATCCCAGCACGTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAG10.6944444444444444No Hit
GAAATAAGCAAAGAATTTGCAGTATATTCTTCCGGGCAGCAGACGAAGAT10.6944444444444444No Hit
CCCTTAATACTAACTGAAAACTCACAATACATATTTTATACAAATACAAT10.6944444444444444No Hit
GTATACACATCATCTCATAGCTTACTTCGATAACTTCTTCTGTATTGGTA10.6944444444444444No Hit
GAAAGGGCGGCAGATGAATTAAAATGAGATAGCATTACCCTCGACTGTGA10.6944444444444444No Hit
GCGAGTGGGTGAGATTGCGCTTAGATTCCCGCCATCTTCCGCATTCCGCC10.6944444444444444No Hit
GCCATGCGATTATTAGAGAATGAAACATCATCCTGGGCATGAGATGACCC10.6944444444444444No Hit
CTTATATGTAGCAGGCTTATACTGTGCCTGGAACAATGGGCTAGGTAGTT10.6944444444444444No Hit
TGTTTGTTGTGTGTTTTGTGTATGTCTGGTCAAATCAATGTCTTTTGTGG10.6944444444444444No Hit
TTATATAGTTGAAGATGGGGCGCCTAGGGAGAATAATTTTGTTCAGTTGA10.6944444444444444No Hit
GAGCTGGAATTACAGGGGCATGCCCCACCGCTCCCTACTCCGCTTAGTAT10.6944444444444444No Hit
GTGCAATGGCACGATCTCGGCTCCATGCAATCTCTGCCTCCCAGGTTCAC10.6944444444444444No Hit
GTATTTTTCTATCTGTGTCGGGCTTGAGTATTTAATTTAAAATGCCTTTG10.6944444444444444No Hit
GTGCAGAACAGGGGTTGTGCCATGGACTGAATGTCACCTACTGCGCCCAG10.6944444444444444No Hit
GTGCAAGTCCTTTACCTTCTCTTTGCTAGGCTTCTTCCTCTTTATTAGTC10.6944444444444444No Hit
GTATACACCAGCCACATCATCAATCGAAACGAGGTGCGCTGAGCGGCCTG10.6944444444444444No Hit
ATCAGCGTTGCTGTGCCCCTCTGTGGATCGTGGGAGCCGGGCGGCCGATG10.6944444444444444No Hit
ACATAACATAACATAACATAACATAACATAACATAACATATAAAATAAAA10.6944444444444444No Hit
CCTGATCGTATTGTTAGGCAAGAATTATTACCTTGCCGACCCCCTGAAAG10.6944444444444444No Hit
AGTTAGTGTCCTTCGGTTAGAATGGGGCAGCAAATATATAAATATTCTAT10.6944444444444444No Hit
ATGCTTGGGATTACTTAATATTATTTCATTGAGTCTGTATGTATAATTGT10.6944444444444444No Hit
GTCTAGTCGCTGCGGCACGGTGACGTGTGACATATCACTGCCCGGAACCA10.6944444444444444No Hit
CCCTTACTTAGCTCCCCTAGCTCGGCTAACATAATGTTACAACCAAAGCC10.6944444444444444No Hit
CCCTGAGATGTCCCTGTTTGTAGACAACTATAACCTATGCACTGTACTAT10.6944444444444444No Hit
TTTCACTCCAGCAAAGGAAAAAAGAGTGAAACAAAGAGTCGGGGAAAAAA10.6944444444444444No Hit
GTATCCCACTGCGGGTGACGATTTATTTTGTTGAGTTGCTCATTTCTGCT10.6944444444444444No Hit
GTATAGTCCATGCCTTCATGTAAAAACACATTTAAACATTTGTAGTCATA10.6944444444444444No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers