FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_negctrl1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_negctrl1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180742
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC553.236146E-452.6471183
CTATACT1157.1848917E-637.768584
ACAGGTC850.002752808234.075228
CTAGTAG900.003647495332.1732373
TGCTAGA1450.001235437924.9550822
ATGGTGT1851.841482E-423.4777666
CTAGACA2303.1605727E-522.0316724
CAATACT1650.00261569321.9362984
CCCTGAA1700.003125457821.2734381
ATGCTAG1750.00369734920.6656251
TAGACAG3208.51549E-720.3596255
TATGAGT1800.004331620420.1082736
CATTAAG1850.005083775619.559392
ATAATAC2659.404968E-519.121833
CAGCACC2359.254432E-418.4824984
CCTGTGT2000.0079611618.0974453
TAATGAG2000.0079611618.0974455
GTATAAA2851.6531588E-417.7651881
ATACTGT2050.009178366517.6560466
TATAGAA3103.1286923E-416.3415552
Produced by FastQC (version 0.11.5)