FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_81_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_81_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178752
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG450.00966691248.2818222
GTTAATC750.001487898938.6254583
TCTAGGA1606.846817E-527.1585272
AAGGGTC1100.00970102726.335545
ACTCACC1450.001230124524.9733548
TGGACTG1550.001813923123.3621715
CCAATAC1902.1992082E-422.8703373
TGTAGAG1700.003102101221.3008042
CTTTAGG1750.003669745820.692212
TATATAC2505.9883285E-520.2783663
ATATACT1800.004320045520.1174264
TCCTTGC1850.00506208719.5737118
TAAGACA1850.00506208719.5737114
TTCGATT1900.005905641219.0586152
CCTTGCT1900.005905641219.0586159
GTGTTTG2050.00915396717.6640821
AAGTAAG2050.00915396717.6640821
AGGAGAG3150.00651665313.7948067
ATTCCAT11653.2057094E-58.0815076
TTCCATT12000.00315336026.63875062
Produced by FastQC (version 0.11.5)