FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_72_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_72_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187752
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGC400.006078368554.2954253
GCATAGG1051.8727496E-434.4457361
GACAGCC900.003637322732.1922267
GTGTTAT1255.2066386E-428.9344161
TATACTG1907.181461E-626.6785475
CAGGACT1100.00970846526.3320714
GTCCTAT1100.009759068526.3040161
CATAGGA1400.00100497825.8549632
TCAGGAC1851.8411437E-423.47913
GAATTAG1700.00312431221.2753071
GCGAGTG350.003659066320.71379140-144
TAATGCT1750.003673221220.6894864
GTATACA1750.00369599520.667441
TAGGCAG1800.004324130720.1147795
ATAGGAG1800.004330813420.1094153
CTCTATA1800.004330813420.1094152
TGAACTT1850.005066866519.5711355
ATATGTG2608.107023E-519.4958635
GTATAAA2659.461279E-519.1076351
GTTAAAG1900.005920326319.0510272
Produced by FastQC (version 0.11.5)