FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_54_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_54_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301598
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC450.00964018948.323753145
GTATTAG2802.6557245E-1028.4339961
ACTATGC1306.52404E-427.8467168
CTATGCA2351.3410845E-624.6473069
ATAGCTC2401.6131944E-624.1298183
GTATAAT3102.4472683E-823.3475631
TAGAACC1600.002188883722.6254584
TAGCCCA1750.003681788520.686135
GCATAGC1800.00433419820.1115178
GTTTATA4001.6520062E-819.9037971
ACTGTGC3001.1143367E-519.3070568
CGAGACC1900.005924921519.0530154
TCTAGGG1900.0059362919.0466962
ATACTGT3051.2851056E-518.9905476
GCCTAAT1950.006896749618.558321
TAACACT2359.2640164E-418.485484
TAGACAG2751.2515284E-418.4294645
TAAGCCT2000.00796580318.1003654
TCTAGGC2400.001068261518.0973633
CTAATAC2400.001068261518.0973633
Produced by FastQC (version 0.11.5)