FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_44_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_44_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204424
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAC1209.596284E-636.2115061
TAGACTC1504.406299E-528.9762975
GTATAGA1504.413667E-528.9692061
GTCCTAC1050.00773432927.589721
GTATACA1855.8344795E-627.4033031
CCCTAAG1358.1107736E-426.8233381
CTAAATC1100.009692471526.3420874
GTATTAG1450.001230702524.9734521
TTAGGGA1650.002606186621.951744
TGACCCT1650.002606186621.951745
TATAGAC1650.002606186621.951743
ACAGTGC2003.1136407E-421.7269048
GTTCTTA2053.6789748E-421.196981
GTGTAAG2053.6789748E-421.196981
TATATGG1800.004315916420.1224295
CTACAAG1800.004315916420.1224293
AAGGGGG1800.00432204120.1175029
TATACAG1800.00432204120.1175022
TATACAC2556.9665504E-519.8856953
CCATGCA2359.231607E-418.4909829
Produced by FastQC (version 0.11.5)