FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_35_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_35_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143838
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC350.003572605662.0887263
TATACTG1201.971166E-742.254835
CTACTAT950.004750262530.4785141
ATAGAGG1306.488742E-427.8603273
TTAACAC1050.007719622527.594993
TCTATAC1050.007719622527.594993
GAGTGAG2156.1470564E-726.9345041
TGAACCC1100.00968563426.3406734
TGTTAGG1100.00970208226.331512
AGTGAGA2552.7089463E-622.717382
ACATTGC1650.00260327621.950568
CCAGGAT2000.00792371118.1092118
CTTTATT3252.204744E-517.8244062
CATTAGA2050.00915358417.661382
GTATTAT2050.00917195117.6552371
TAATTCA2500.001393658117.3848444
TATTATA3403.2484797E-517.0380362
TATTCTT2950.00420633814.7329185
TATATAC3456.993144E-414.697333
TTATTCT3456.993144E-414.697334
Produced by FastQC (version 0.11.5)