FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_23_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_23_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122643
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT800.00205411336.161671
GTTGGCC1000.006077821428.9766258
CTATGGT1000.00608997528.9647884
AGAGTAG1306.496234E-427.8507585
GTCTGGG1100.0096963526.3316257
TATATAG1100.0096963526.3316255
TAGTCTT1100.0096963526.3316255
ATACCAT1400.001001517625.8614186
ATAATAC1400.001003909625.8508593
ATATAGT1450.001237789624.9390831
CCATTCG1751.2537885E-424.8371079
TAACACA1801.522174E-424.1373214
TATTGAA1550.001820880823.3396322
TATAATA2303.162303E-522.0204352
CTTTCTA1750.00365771920.6975889
TAAAGAC1750.00366639720.6891355
TAATACA1800.004316119520.1144354
TATTATA1950.00688744418.5520152
TACACAT2000.00793286418.1029935
ACACATT2500.001395265617.3788726
Produced by FastQC (version 0.11.5)