FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137116
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT553.229367E-452.6603624
TAAGTGG604.9610133E-448.2719965
ATAGTAC604.9610133E-448.2719963
GTATTAT700.001061955441.3608971
TATAGTA907.504572E-540.2266652
TTACACC1157.159817E-637.7780844
GTATATA900.003646472532.1695861
CTTACAC1153.1715058E-431.4817373
TAATACC1000.00609410628.9631984
TCTAAGC1100.00970289626.3301813
TTTACAC1450.001230116724.9682753
TAGAAGC1450.001230116724.9682754
CATTATA1500.001498753324.1359982
ATATTCC1550.00181391823.3574184
CTGCTCA1902.198597E-422.8656834
GTATTGG1650.002614223921.9338071
TGGAGGA1700.003102108821.2964695
GGCTGAA1750.003669760420.6887
TAAATGG1900.005905696719.0547354
ATTCTAG1950.006875749718.5593761
Produced by FastQC (version 0.11.5)