FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAATCACACGAACGGAGTATTAGGATGGAAATCGAATGTTGAAAAA12.1739130434782608No Hit
AAAGCGTATCGTTCAAAACCTGTCATTTGTTTATCAAAGTTATCAGTTAG12.1739130434782608No Hit
ACACACCTTGGCCTCCCAAAGTTCTGGGATTACCGGCAGGAGCCACCACG12.1739130434782608No Hit
CCAATTTTAATATATTAAGTGTCCAACCTGCTACGTTTGACTTGTAAACA12.1739130434782608No Hit
GGGGACAGTATGCCCTGGTAAATTTGGACCAAGTTGAGTAATTATTTGGC12.1739130434782608No Hit
GAATTATACACACACGCACGGTGCTGGTTAGCATGCCTTTCATCACACCA12.1739130434782608No Hit
CTACAGGGACGCTGAAAGAATAAATCAATTCAGCAATTATGAAGTTATTA12.1739130434782608No Hit
CTAATGACATTTCAAGAGCAAAATCCTACTAATTCAAAACTATCACTATA12.1739130434782608No Hit
GTAACAACACTTTTAATAATAACTCTAAGTACTTGTTATCGAAAAAAAGT12.1739130434782608No Hit
GTTCTAAACTTTGTGCTTTCAGTTCTACACACATACATTAGTGAGTTGGG12.1739130434782608No Hit
GTTAATGATGTATCCGTGAGCGGCAGGATACTTTAATCGACGGGATCACC12.1739130434782608No Hit
AATTCAACCGAAAGTTAGTTTGAAAAATACAGCGAAAACTTATTTAAAAA12.1739130434782608No Hit
GCTCGATAGCGGTAGGGGCATGGAGTATCATCCGATGAGGAAGTAACAAA12.1739130434782608No Hit
CATGATGATACTATTTGAGACCATCCAATTAGTACCTTCGATGCAATTCG12.1739130434782608No Hit
GACAATGAGACAAAAATAAAAATTCAGTTGTGTTGAGTTAAACGAGAAGT12.1739130434782608No Hit
GATCTAGAGCGACGGGGGGGGGGTGTTGTGGTTGGTTGGGTTCGGGGTGT12.1739130434782608No Hit
GTCCTATACTTTTTGATTTTAAGGCCTAATGGTTGGTATAAGGTTGGCAA12.1739130434782608No Hit
CCACTTGGGGTGTCCATGCTATCCACTTAACTTTGTAAACATAGTAAGAT12.1739130434782608No Hit
GGTTCGAATTCGCCCCTGCAAACTGGCACAGGACCACCAGCCAAGGGCCT12.1739130434782608No Hit
AGCAAAACCATCATTTCACTCTGTAATATATCGTCAGTTAATCCTGTTTC12.1739130434782608No Hit
CGTGTGAGTGGTGGTTATTGGTCAGTTGCTTATAACTAAGCCCTCGCTAC12.1739130434782608No Hit
CGCTCCTCCTTCGTTGCAGTTAACTTAGAGAACTTCCCAGTAGGGCGAAG12.1739130434782608No Hit
GTGAGGTTGTGGTTAGTTTGTTTGTTGGATTAAGGGGGTGTACGGTTTAG12.1739130434782608No Hit
GTGCGGTTGGGTGGGGAGGAAGGTAGCGGGTGGCTGGCACAGCAGGTATC12.1739130434782608No Hit
CCCTCCCAGGAGCTGGGACTACAGGCCCACACCACCCTGAACGGGCAGGT12.1739130434782608No Hit
TATTAAGCAGGTGGTTTTGTATTTTGCCCCTTGTCCTACACTACTGCGTT12.1739130434782608No Hit
CCGCATAGCACTCCATTTCCTAATCTAGACATTTGCCGAGATATGGGTAT12.1739130434782608No Hit
GTAAAATGTAATGCTCAAAAGACGGCTGGGAGGTTGGTTATTTAGGAGTA12.1739130434782608No Hit
AATTAATCAAGATAATAAAAGAAGATGAAAGACAAAAGCCTTAATCACTC12.1739130434782608No Hit
AAACATGTTAATAGTTTTATAATTTTTATCTGCGAATATTCACATAATTA12.1739130434782608No Hit
GACATACACAGGTTTAGGCTTGTCTGACGCAATGCACATGTGGTAGCAGG12.1739130434782608No Hit
TCTCTTCAATGATCAAGAAGTTATCCAACTCTTTACTGGTCAATTCATAG12.1739130434782608No Hit
GAGCATGAACTCCCGTGCGTTTTGTGGCGCGGAGAAATTTTCTACACGCT12.1739130434782608No Hit
GATGGTGATTGCATATTTCACTGATAAAAAGTTGTAAGATTGCTCTGCAG12.1739130434782608No Hit
CTACATCTTGGCGCTCTGTTGTCTTCAGAGACGTGCATTTCAGTGTGGAT12.1739130434782608No Hit
GGGGATGTTATAGGGCAGTCGCTTCACTTGCCGGTGTGCTAAGCCTATGC12.1739130434782608No Hit
CTGGAGTGCATTGGTGCCATCGTGGCGCCCTTCAACATCAGTCGCCTGGG12.1739130434782608No Hit
GGGTATGCTGAACCAGGGTTAACATGGTAGGGTGCAAAATCGCGTTGTGA12.1739130434782608No Hit
GGGCTGCGCTGCACTGGGGATGCTGGTTGCACGAGCGCGTCCAGTGAATT12.1739130434782608No Hit
TTTCTATGTTCTGTACCTACGAGTCTACCCATGGTTCCTGCATATGCCAT12.1739130434782608No Hit
GTCGAAAAAACTGTCATATAAAGTTTGTAAATAATCCAAGGTCTTATCCT12.1739130434782608No Hit
TCAGAGAGGGATGTCTAAAATATTTTCATGTAATGAAACCACTGAAGTTA12.1739130434782608No Hit
TAAATTATTTTTAAATCCCTACGAGAGAGATATTCATTTATGTGAATTGA12.1739130434782608No Hit
TGCCTGGATTCAAATACTAGCTGCAGTATTTACTTGTGTAAGCTTGAGCA12.1739130434782608No Hit
CGTAATACGTAGCTGACGACTTGTCTGAAGGATTGCAAATAAAAGTGCAA12.1739130434782608No Hit
TTCTTATACTTTACATCATTACGTACTTCATTGGAAAGGTTTACTTATTT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)