FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151483
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAC553.231584E-452.656643
CGAGACC657.3532783E-444.570344
CCTACTC950.00474578130.4854183
CTAGGAG950.00474578130.4854183
GTGTATG1000.006108066528.9515861
ACTAGGA1050.00773859727.5820472
TTAGCCT1100.00969377526.3370194
CTTAGAG1100.00970940926.328323
GTATTCC1400.001005289525.8496321
TAGAACA1751.2574489E-424.8320474
ATAGAAC1500.001500098824.1342933
TAATCTC1550.001812054323.3634835
CAAGACT1650.002606005921.9475154
GTGTAAA1650.0026160321.9330181
TATCTTA1700.003098924421.3026
ACCACAC1700.003104873721.2949623
TTAGAGA2104.3202128E-420.693374
TTAGACA1850.005056935419.574814
TTCTAGA2000.00794707318.100722
ACTGTGG2050.00914472217.6650738
Produced by FastQC (version 0.11.5)