FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_21_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_21_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279617
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT2301.1125248E-625.1803364
ATACTGT3554.154572E-922.4357956
CGCCGTA350.003647319320.726152130-134
GCTAGAC1800.00434518220.1020983
AGACTGT2908.306761E-619.9741886
ATAGGTA1900.005921503519.0543236
CTACCCT1950.00688670518.5624284
GTTCTAG2359.3244755E-418.4669061
CAATACT2751.2521703E-418.4274254
GCACAGT3201.9413043E-518.1016086
CTAAGAC2000.00798591818.0918883
GACAGAT2050.00916895817.6632677
ATGATAC2050.00920686917.6506213
ATAATAC3302.5410161E-517.5436483
ATATACT3352.8824676E-517.2879924
TAATACT2550.001602814917.0337564
CTTCTAT2550.001612449117.018521
CCTGTCC2700.00234027216.0960838
GACTGTG2750.002648268415.8005957
CAAGACA3706.7514135E-515.6526424
Produced by FastQC (version 0.11.5)