FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177366
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG1501.1401135E-633.783011
CCTAGCA1301.660804E-533.421192
CTATAGT1306.5075216E-427.8509924
ATAATAC2105.000038E-727.5857433
ATGTACT1550.001815351223.3588964
GCTCTGT3157.296603E-720.6893086
GTATAAT2104.3382408E-420.6834751
TACAGGT1800.00432343120.1146074
GTGTAGA1850.005074324619.5654491
TAATACT1900.00591026219.055944
TCCTAAA1900.00591026219.055942
GTGTAAA1900.00591990319.050571
CTCTAGA1950.00687774618.5620941
GACTGGG2050.00916110917.6616047
GTATTAT2050.009176007517.6566261
TGCTCTG3103.1142106E-416.3512295
CTCCTGG5002.0984407E-715.9262761
TTGCTCT2850.003355617415.2447544
TATAATA3951.1725905E-414.6658372
CTGGGCT3558.719039E-414.2785374
Produced by FastQC (version 0.11.5)