FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTATGGAGCTAACTACATTTTCTTAGAAACAGCATTAAAATTAAA30.24671052631578946No Hit
GTCATTAATCCTTCCTTCTACTGTTCAGTCTATGTCATATCAAATAAATT30.24671052631578946No Hit
CACTTGCAGATTCTACAAAAGAGTATTTGAAAACTGCTCTATCAAAGGAA20.1644736842105263No Hit
ATGAATTATAGTAGATTTCATTTCTTCCTATTCTGAATAGTCCAATCACA20.1644736842105263No Hit
ATCCATTTGTAACCATGGACACAATAAAACCATATTCTTTTGCTTTTTTT20.1644736842105263No Hit
ATGAAACTGGATCCTCATCTCTCACCTTATAAAAAGTCAACTCAAGATGG20.1644736842105263No Hit
GGACTCGGGGACGGTCCACAGGGCGTCGGGCACCATGGCCTCGAAGCTGC20.1644736842105263No Hit
CTTCAAAACAGATACTGACGATCATGGCTTGGCGGGTGCGTGTGACATCA20.1644736842105263No Hit
CAGCATCTCTAACCTTATCCCAGACATTTGCCAGTGAGAAACAATACAAG20.1644736842105263No Hit
AATGTACCTTCCAGGAGAGCTCCGTATTGCCTTCCTTCTCCTCTTTGTCT20.1644736842105263No Hit
ACCGAATCCAGCAGCACATCAAAAAGCTTATCCACCATGATCAAGTGGGC20.1644736842105263No Hit
TTGGTGGCCTTAGCAAGAGCAGTTATATTGAATTTGTTGGGGTGGAAGTC20.1644736842105263No Hit
GGCTAATTTTTCTATTTTTTGGAGAGACAGAGCTTTGCCTTCTTGTCCAG20.1644736842105263No Hit
CCTACAAATAGTCTTTCCCACAATCAACAACCCCCATCAGTGGGGTTCAT20.1644736842105263No Hit
TTGCCATCTTTGTCACTTACTTTTACGTGCTTAGCCGCAGAGGCAGAGAG20.1644736842105263No Hit
GGCCTGGGGCCGACCCAGCCCCGCGCCCCCGGAGCTGAGGTCTGTGCGGT20.1644736842105263No Hit
CGAGTCACTCGCAACTGCACAGTCCTGAGTGCACCCATGTGGCACACTTA20.1644736842105263No Hit
GCGATATATCTCTGCACTGATCACCTAGGTCACGTAACTTTTGTCTAGGC20.1644736842105263No Hit
ACCTTAATAATGAGCTTATCACCATCCTTAGAGAGGTGAACCCATGATGC20.1644736842105263No Hit
CAATTGGTACCCAGTGAATGATTTGGGGAGGGGAGAAGTTTAGGGTAGAA20.1644736842105263No Hit
GAATAGGTGAAGAGGTCATTTCTGCTTACGTCCATAAACTGCGCAACTTC20.1644736842105263No Hit
CCAGTATGTAAGAAGGATGTTCTTCAAAACATGTCTTTGTTTTGTTTTGT20.1644736842105263No Hit
GCTTGAATGTACATGTTGGTCAAACATTATAGATTATTTCTCCAGTCAAC20.1644736842105263No Hit
GTCAAATTGTATATGTTTATTCACTTCATGAAATAGCATGACCAAGGATG20.1644736842105263No Hit
GTGTATATATGTGTGTGTGTATTCCCTAGCTTTAGGGGTATACAACTGTG20.1644736842105263No Hit
GTATATTGTGTCTGATACCATTTGCTAACAGTGATATAAAAATGCATGTC20.1644736842105263No Hit
TTGGAGCACTTTGAGGCCTATGGTGGAAAAAGAAATATATTCCCATAAAA20.1644736842105263No Hit
CTTCAGAATTCCCTCATTCTGAAGCGTGTCCTTTATCATTTATTATCGAC20.1644736842105263No Hit
GAGGTTGTCAGGTTTGAACAAGAATTTGGTCCATATGTCCATAGGTAGAA20.1644736842105263No Hit
GTCCTAGATGAGGAAACACATGTGATCCCAATTCAAATTCAGCAACGTCT20.1644736842105263No Hit
TTGGGAGGCCAAGGTGGGCAGATTGCTTGAGCTCAGGAGTTCAAGACCAG20.1644736842105263No Hit
ATACATGTCTGTATTTATGTGTCATGTATATGTTTCATTATCAAAGATAT20.1644736842105263No Hit
CAATTTGTGTTGTGCAGAGATAACAAAATATGCGTAAGAGAAAGAAGAGG20.1644736842105263No Hit
TCATACTTCTTCTTAACCTGCATAGGTTTAAATTTCTTCTCAGCCTGCTC20.1644736842105263No Hit
CAATTCTACCTTGTAATCTTCGTTTTTAGATTTGAAGAACTCGATAGCTT20.1644736842105263No Hit
GATTTATTGCATGACGCCACTGAGGTTTGGGATCTGAGTGATCCTATCAC20.1644736842105263No Hit
GTGCATATCACCTAAAATCTCTTGATAAGCTCCAACAAGGAAGACACCGA20.1644736842105263No Hit
CTTAATCACTGCATACATAGCTGGTGATGGTTTTACACTCTGCTGAGCTT20.1644736842105263No Hit
CACCTGAAAGTAGCCATTTTTTATTATCTTTATCATATTTTCCACTCTGT20.1644736842105263No Hit
GAACCAATATTGCTAAAAATCAAACACAAAATTTAATATGTGCAGCAAAA20.1644736842105263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAA100.006238093145.02
Produced by FastQC (version 0.11.5)