FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134992
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTG350.003568594662.1042255
GTACTGT850.002756965334.058541
GTATTAG1301.6638693E-533.403571
GACTGGG950.0047273130.507347
TATACTG1204.0543728E-430.1895565
ATTATAC1504.38946E-528.9819743
CTCATAG1000.006107627428.9497591
TACTAAG1050.00770855627.601882
CTCTTAG1050.007750534527.57121
AGGCCAG1100.00967176926.3472485
TACTATG1100.00967176926.3472487
TGTTATA1700.003090221721.3102742
TATACAG1700.003090221721.3102745
TCATATA2000.00790983718.1137312
ATATACA2900.003746542114.9906764
AATTATA2950.004197568614.7365962
TTAGACA950.007702371610.68549555-59