FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222483
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT1251.2700826E-534.762234
TATACTC1251.2700826E-534.762235
TCTATAC1501.1382472E-633.7966163
CTATAGC950.00477603930.4520571
GTGTAGG1000.00613703628.9294511
GACTGTC1050.00772814427.5952877
CCAGATC1450.001231209324.9728683
TAGTTCC1600.002177889522.6418579
GTATATA3304.7701178E-821.9162521
AAGCTAT2003.1105775E-421.7312896
CAAGACC2755.2295636E-621.068024
TAGGCTG2155.076155E-420.21065
TAAGCAG2205.9267395E-419.751275
AGCTATC1850.00505987619.5777387
AGACTGT2256.884433E-419.31676
TATACAG1900.00591074519.058245
ATACTGT2050.00914994817.6677136
ATGTAAT4155.3318763E-717.4273821
CACACTG2500.001398917517.3811155
GTGTATA2800.00301017215.497921
Produced by FastQC (version 0.11.5)