FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGCAGGCCCTGTATCTGAAAGAGGACATACATTCCAAGTTGCAAAGA11.2658227848101267No Hit
CTTTGCCGTATAACCTGTATTTAATGGTTTAGCTTATGAGTGTATCCAAG11.2658227848101267No Hit
ATTTATAACATCATATGAATTGTTGAAAAGAAAAGTAATCTTATACATCT11.2658227848101267No Hit
AGACAGAAGAATTCTCAGTAACTACCGAGTGTTGTGTGTATTCAAATCAC11.2658227848101267No Hit
AAATAATGTTGGACATTTCGTGTTCTCGTTCTCTCCTGGCCCCCTTGTGG11.2658227848101267No Hit
AGTTTTTAATCTCTCTACAAGGGTTTTTCCTAGTGTCTTAAGAGCGGTCC11.2658227848101267No Hit
ATAAAGGTATCTGAGTATCTCTTTATACTTTACAGCTTACATACCAGCAT11.2658227848101267No Hit
ATTGTATATTTAGAAAACCCCATCATCTCAGCCCAAAATCTCCTTAAGCT11.2658227848101267No Hit
GCGTAAAAAGCTGGAGGAAACATGCCTATCACCCATTTGGATATAAAGGT11.2658227848101267No Hit
TGTATTTAGTGCTTAAAAGGAAAGAAAAGGGTGCTGTGACGGTGACTATC11.2658227848101267No Hit
ACCACAAGAAAATTGCAACTGGGCTCCAAGCCTCTGATCTATCACAATGC11.2658227848101267No Hit
TCAATAGCCATGGGAGGTATGAAAGTGATCACATTACAAGGACTTTAATA11.2658227848101267No Hit
GTTTTTATACATTAGATAGTTCTTGTAATTGTAGGATGACTGTGTGACCT11.2658227848101267No Hit
AGACGGCGTCGCTCTCTTCTCCCAGGCTGGAGTGCAGTGGCGCCCTCTCG11.2658227848101267No Hit
TTTATCACGTTTTCTAAGAAAGCATTTAAAAAAACAGTTCATATACCAGC11.2658227848101267No Hit
GATGTATAATGCCTTTCACAAAAGAAAGCTGAAATAATTAATAAGCATCA11.2658227848101267No Hit
ATAAGTAGGGAAGTGCGACCACTCAGATTAATTAAAGGATGGCATTCAGG11.2658227848101267No Hit
ACCTGGAGGCAACCAAGGATTGGTGCTTGAACGCTCTAAAGCAATAGGCT11.2658227848101267No Hit
GGAATTGCCACTTAGTCCTACACATTGAATGCAGAAAATTCTGATGGTTT11.2658227848101267No Hit
ATGCTTATCTTAATATTACAGTTACTTCATATGGTGTTATGAAGTTTAAA11.2658227848101267No Hit
CAGCAATGGATCCAACCCCTTGTATCCGGCTCATGCAGTAACCTTTGACG11.2658227848101267No Hit
GGCAACTAAGGGAATAAAAGATGCCCTACACCAGCCATCAGCTTGACCAC11.2658227848101267No Hit
TAGTGAAACACTGCACTCCAGCCTGGGAGACAGCGAGCGCCGGCGTCTCA11.2658227848101267No Hit
AAACAACTGGGAAATTACGCTCATACGGTGGGACCGGTCCAAGAGGGAAT11.2658227848101267No Hit
GCTGATGCCTCCCGCCTTGTGTATCTTCCTCCCAGGGCGTTTTTTTAGTG11.2658227848101267No Hit
TGGTAGGATTCCGGCACACCTACCTGAATGGCACGAGATGTGCTAAGTAT11.2658227848101267No Hit
ATTCAATTCCATTCGTGTTTATTCCATTTCATTCCATTCCATTCAATTCC11.2658227848101267No Hit
ATTCATTGGATTTTGAAAAGCGAGAATCACGGAATTTTCCAGCGGGAAAG11.2658227848101267No Hit
AAGCAGAATCTACAATCCACAATTCTACAGAGAAAAATAATTATCCTCAT11.2658227848101267No Hit
GCATTCATGTGTGTTTTTACCACTAAAAATAAGAAACTACCTTTCAATGT11.2658227848101267No Hit
AATCAGAGGATTATTTGCAACACTAAGCAACCTCCCCCAGACGCCATCAG11.2658227848101267No Hit
GTGTGAAAGAAGACCGCCATATCCAGGAAGGCAACTTTGATGGTGGGCGG11.2658227848101267No Hit
CTAAAAATCAGAGCACCTCTGGTCCTCCACAGAAACACACCTGAGCCCGG11.2658227848101267No Hit
CACCAACGCTAGGTACCATTTGTGTATGTGTGGGAACACACAACACCTAA11.2658227848101267No Hit
GGTAATATTTTATTACTGATTTTGACTGCTTTTATGTTTAATTTGTTTTC11.2658227848101267No Hit
ATCCTGGCTAACCACGGCAACCCCCCACTCTCCTAAAAAAACAAAAAAAA11.2658227848101267No Hit
CTCTACAACTTTTTATTGATTTATCAACTGCATATAAAATATTCCATGGT11.2658227848101267No Hit
GGAATCAAGTACCTGGCGGTAGCTGCGAGGTCCCTCTCGCACTATCCCAC11.2658227848101267No Hit
CTTTTCTACCATTGTCCTCAAAGCTCTGAAATCTCCACTTTCAAATTCCA11.2658227848101267No Hit
AGCTTTCTGTTGGAGGAAGAGACAAAAAATCCTGATGTTCATGAATATGG11.2658227848101267No Hit
ATTCATCCGTAGAATAGGGATGACGGTTCGGGTCTTAGGAGAAAGGAGAT11.2658227848101267No Hit
GTTATGAGTAAAAAATAAAAGGGCAGATTAAGTTCGTGCCCCGAGAATGT11.2658227848101267No Hit
CCCGAGAATAAGACAATGATCCCCACTCAGGCAAAATCTACACCAGCCAC11.2658227848101267No Hit
GTACTCGCTACTTAAAAACCTACGTTTTTACATTTATGTTATGAGTCATT11.2658227848101267No Hit
CCCCGTTTGCCTTCCCAATATTCAGGGTTAGGATACAGCATCTCAAGGCC11.2658227848101267No Hit
CATTATGGTACTTCTTGCAACCTTTCAGCCATGATTATTATATCTACTGT11.2658227848101267No Hit
CTTACTCAGTATTATACTTTCTAGCGTTCTATGAAGAAATCCCGTTACCA11.2658227848101267No Hit
ATGCAAACAGATTAGAAAGCAGATAATACAGGAGAGGTTTATAAAATTTG11.2658227848101267No Hit
CTCTTACTTTGGGCTTACCCTTGAAACCAGATGCCGCGACAGGTCGCCTG11.2658227848101267No Hit
CCATAGCACTTAAAACAAGACAGAGCAGTTTCCCTTCCCCAGGTACTACT11.2658227848101267No Hit
GAGTCTCCCGAGGTCGCCCGGGCGGGAGTGCAGTCTCACGATCGCAGCAC11.2658227848101267No Hit
CTAATAATTGAAAAGTTTTGTGATTGATAAATACCATTTTGTTATGTTCA11.2658227848101267No Hit
GGCAAGTCGCCGCACGCTCGGAGCTTCTGGTGCCTAACCCCTACAATAAA11.2658227848101267No Hit
GATTTGAAGATTTCTTTGGAAACTTGAATATCTTCATATCAAATCTAGAC11.2658227848101267No Hit
AAGTGATTTGGGGTCACACCAGAGCTGGAGCAAATCCAGGAGGTACAACA11.2658227848101267No Hit
CTCGTTCTCTGCCTACCTCTGACTACTATTTTGGGGGGCTTACAGGCCGG11.2658227848101267No Hit
CAGAAACCCAGATCAGCTCAGCATCAGCAAAAAGGGAAATTTCTCAAAGA11.2658227848101267No Hit
GAGCAGAGGGTCCTGGAGCCCCAAGCAATAGAGTTTTCTTAATTGTCCAC11.2658227848101267No Hit
CTGCACTACAAGCATCAAGCTATTTTTTTGTGTGGGTTATTGTAGAAATG11.2658227848101267No Hit
GTGTAAAAAAACAAAAAAAAAAAACAAAAAAACCAGACATCTAACTACAG11.2658227848101267No Hit
GAGTAAGACAAGAGACTTGGGGAGAACCGAAACTGTGATTTTGGACATGT11.2658227848101267No Hit
ATATTTGATAACTGATACACTTTCTTCCAGATGAATGAATTGGCTACTGA11.2658227848101267No Hit
GTCTCAAGGTACATCACTCCAGATTTAGGAAAATAATATTCAGTATAGAT11.2658227848101267No Hit
ATTAAATGATTAACCAACATGAATGAAAGTTTACCAATGGCAGCTCATCT11.2658227848101267No Hit
CAATATTTGCGTAGGGCCTCACCGGCCATGTTTGGGTCTTGGGGATCGAG11.2658227848101267No Hit
ACTTGTGACTATGTGAGTCAATTCGACTTAATCTATACATCCATCCTACT11.2658227848101267No Hit
AAATATAAGAGAGATCAAGGACTAATTGTATATCTGTGACTCGAAAACTA11.2658227848101267No Hit
GACATGAACTGCTCATTTGTTATGGCGGCCGAGTTTTGCATGGTGTATAT11.2658227848101267No Hit
TGATGTGGCGCTTCTTCCTGGCTGTATGGCCGTGGGCCGTACCCGCTAAG11.2658227848101267No Hit
GAGTAATATTGTGACAGGTCATATCATAAGTTCTCATAAGCCTTTACCTT11.2658227848101267No Hit
GTGCTTAAGTCAAGGAGAAATTCTTTCTGCTTGTCCTGAAGAGCGGTTCT11.2658227848101267No Hit
TTCCACTTCTTTTAAATTACAAATATCCTCTTGCTATTTATTTCTTCTTG11.2658227848101267No Hit
CCAAAGTCTCGAGTCTAAATGTGAATGGACTCTGGACCCAGGCTGCTGAG11.2658227848101267No Hit
CGATATCCGTGCGTGGGAAGAGCTAAGGGGAGTGAGAGTTATTGCGTGAG11.2658227848101267No Hit
AATGAGCCAGGTTTGTCCCGCACCCCTCTCAGGTCCGGCACGGGAGATTC11.2658227848101267No Hit
CTACTAAAAAATTTAAAAATTAGCTGGGCATGGTGGTGTTTGCCTGTGAT11.2658227848101267No Hit
GGTCATACCTCTGATCGACTCTGGGAATGTTATACGTTCTTAATTCCGCC11.2658227848101267No Hit
AATCAAAAGCATATTTTGATACATATCACCAATTATATGGAAACATAAAT11.2658227848101267No Hit
GGGCAACGGGCCGGGCACAGTGAATCATCACAGTAATCCTAGCCGTCTTG11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATTG50.0145.02
CATTGGA50.0145.04
GGATTTT50.0145.08
ATTGGAT50.0145.05
TGGATTT50.0145.07
GATTTTG50.0145.09
TTGGATT50.0145.06
TCATTGG50.0145.03
ATTCATT50.0145.01
CGACTGC50.0145.0145
Produced by FastQC (version 0.11.5)