FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAACTCTGTGACTTGAATGTAAACATCCCTAAGATGTTTCTTAGAAT30.18808777429467086No Hit
AACACCTCCTGTCCGTGGCGCTGACGGCTGAGGACAGAGCTTTGGTGTGG30.18808777429467086No Hit
GCTAAAGTAGTGTTAAGAGGGAAATTTATAGCACTAAATGCCCACATCAG20.12539184952978058No Hit
GTCTAGAGTAATAAAATTGTAAATGCCTGGGTTTGGTGAATAATAGTTAA20.12539184952978058No Hit
ACTGGATAGTGTGTGAATAAGTAAGGTGACACTGCCAAATATAAAAAGCT20.12539184952978058No Hit
GGCTTGGTACAGCCATGGTCGAGGCATGTTTGTCTTTCCTCCCCACCTGG20.12539184952978058No Hit
GCCATCAGCAATGTATGTGAATTACGTTTGCTCTGTGTCCTTGCCAATGT20.12539184952978058No Hit
CTGATATTCTGCTAGGCCGATGATAGAATTTGGATGTGCCGCTCTCATGG20.12539184952978058No Hit
CTCCAGGTTCAAACGATTCTGCTGCCTCAGCCTCCCTAGTAGCTGGGATT20.12539184952978058No Hit
ATTTATAAAGTTCTTAGAACAGTGCCTTCATGTAGTGGGTGCTGTATAGG20.12539184952978058No Hit
GTGTACTGGGCCATTGAGATGAAGTCACTAGAGTTCATTCATTCCTTGCT20.12539184952978058No Hit
GATAAAAATAGTCCAGGAGAAATCTGAGGAATTTTAATTGCTGCTCAATG20.12539184952978058No Hit
GGCCTGCTACAGAATGCTTGAAGTTAGATTAAAAATAATCTACCGATAAA20.12539184952978058No Hit
GCCCAAGCAGGAAAAGGCCAGAGCTTAGATGTTCTGGAGAGTACCCTGGT20.12539184952978058No Hit
GTATTGAGAATATAATAACGAAACCTCTGTTTTCAATAACTTTGAGGGAA20.12539184952978058No Hit
GTAGAATGATTTATAATCCTTTGGGTATATAGCCAGTAATGGGATTGCTG20.12539184952978058No Hit
GAACATTTTAAAGCAATCATTGACATTTAGGAGGCATATATTCACTAAAA20.12539184952978058No Hit
GTGCAAAGCTGCTGAACCCATGACATTTTGCCATTAATCTTTTAGTTTTC20.12539184952978058No Hit
AAGGTAGACTAGCATATAGTTCATGTGCTCAAGGAGTTCATTTTTATTGA20.12539184952978058No Hit
GTCCCAAATCTGAGAGGCTTGTGTTAAATATGCAAGAAGTAAATTAGTAG20.12539184952978058No Hit
CCCCAAGTCCCTAGGCTGCACAGAGCAGGGGAGACCCTGGGCCTGGCCCA20.12539184952978058No Hit
CAATATACTGTCACCAACCTGGCAAGGCCATGATCAACTAAATCAGGGGA20.12539184952978058No Hit
ATTGTATACTGTACATAAAATATCAAAGTACCCAAGGTATATATTCTATA20.12539184952978058No Hit
GTTTGCCTGTCACTGGAGTTAATGTTTCCTTTGGGCTTTTTTTTTTTTTT20.12539184952978058No Hit
ATCTGATTCCATTCAATGATGATTCCATTTGAGTTTATTTGATAATTACT20.12539184952978058No Hit
GTCTGTTTCTATTAGTTTATCTTTAGCATTAACCTATGTTTACCAAATAA20.12539184952978058No Hit
CTTTTACACTATGTAATTTCTAAAATATTTTGTGGTTACTTTGAGATAAT20.12539184952978058No Hit
GCTCTACCTCCTGGGCTCAAGCCATCCTCCCACCTCAGCCTCCTGGGTAA20.12539184952978058No Hit
TCATTGTAGCTTTGCTGTTAGTTTTGAAACTTCCTAGTTTCAAAACTAAC20.12539184952978058No Hit
TTATAGTAGTGTGAGAATAGACTAATACAGGTTTTATGTGACATTTTATG20.12539184952978058No Hit
GGAATTATGTGGTAAATTTTTCTTCTCACGAGTTAAATGAGATTTAGGCT20.12539184952978058No Hit
GTCTGGTCTTGAACTCCTGGGCTCAAGCAATCCTCCCACTTCAGCTTCCC20.12539184952978058No Hit
GAGTAATAGAGCTCACAATGAACTCAGTAAGAATTAATTGGTAAATGGTG20.12539184952978058No Hit
GGCAGAGACACAATAAAAAAAGAGAATTTTAGACCAATATCCCTGATGAA20.12539184952978058No Hit
GTGTATTTGTTTAAGTGATTTTCAGATGTCACTTCATGATGCTTTGTAAA20.12539184952978058No Hit
GAGTTGAACCTTTCTTTTGATTGAGCAGTTTGGAAACAGTCTTTTTGTAG20.12539184952978058No Hit
CTGGGGCACTGCCCACCTCTACATCAGAGACCTCCCTCACCCAGGAAGCC20.12539184952978058No Hit
GATTAAAATAAATTCTTTAGTGTCTCTTTTCTAGATTGACTGTGCCTTCA20.12539184952978058No Hit
CATACTGGCCACACTCGTGCCGTGTGTGCAACACGCACCATGTTTCACGT20.12539184952978058No Hit
TTGCAGTTCAGCTGCTTTCCTTGCCTTCTCAATGGCATCATTCATGAAAG20.12539184952978058No Hit
TTGTGTAACACTGTAATTTGAATTTATAGCAGCGTAATTTCAGTAACATG20.12539184952978058No Hit
AGTCCTAATATCTGATCCCATTTTATCCAACAAAACTTTTACCCTTGTAA20.12539184952978058No Hit
GTCTGAAGCATGCTTTGCTTAAAGAAGTATATTATTGTATTTAACAATTT20.12539184952978058No Hit
CATTTATAGAGCACCAGATGTTTGCTTCCTCTGAGGGTATCCAGAAAGTC20.12539184952978058No Hit
GACTAGGAAACTCAACCAGGCAAGTTAAAACTATATTTCCAAAAAGAGGA20.12539184952978058No Hit
CTCCTGAATACAGCACACTGATGGGTCTTGACTCTTTATCCAATTTCCCA20.12539184952978058No Hit
GACCGTAAATCTGAAAGATTTTCACAGTGCACAGCCCATTCTCAGAGCAC20.12539184952978058No Hit
TTGAAGAGTGGAGTGATATGGCTACTAGAAAAGGAATGCCAGCAGCCACC20.12539184952978058No Hit
TAGCTACACAGGAAAAATATTAAAAGGTTACTAGAAAAACACGCATCAGG20.12539184952978058No Hit
GTATATGTCCCTCTGACCACATTGTAGCCACTCAATCAGAAGACAACCCC20.12539184952978058No Hit
CTGTAAATAAATATAAAGTTCCATAAATATTTAGTGCCTACCCCTGTAGA20.12539184952978058No Hit
GTTTAAAAGTGTGTAGCACTTTACCCCTTGCTCTATCACTTGCTCCTGCA20.12539184952978058No Hit
ATTTAGCAGGAATGGAAGACTTGGCTGACACTGCTAAGACCAACAGCTTC20.12539184952978058No Hit
GTTGTGTCTTTACCAGGTTTTGGTATCAGAATGATGCTGGCCTCATAGAA20.12539184952978058No Hit
AATGTGGGGTTCTTGGATGACGTATTCTGTAGATGTCTATTATATCTGAT20.12539184952978058No Hit
GCAAGGAAATGACCTGAAACTGGAACTTATTTAAAAGGGAAGCAGAGCAT20.12539184952978058No Hit
CTATTGGTTAAAGCCAAGAAATTAACACTGTTCATTCTTCTCCTCCCAAA20.12539184952978058No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)