FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_14_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_14_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223425
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA450.00966215448.291819
CAATACG450.00967071248.2809874
GTCCTAA1153.1869404E-431.4664541
GTATAGG1000.00611672928.9491391
ATAGTCT1555.5137665E-528.0404056
GTATTAG1855.8692167E-627.384321
TCCTAGA1450.001236045624.9561542
TATAGTC2101.5583855E-524.1459035
ACACTGT3253.9910447E-822.2885276
TTAGACT1952.625107E-422.2835334
CCTAGCC1650.002617771321.9360753
TACACTG2053.6754087E-421.201285
TTACACT2053.6809777E-421.196534
GTTTATA2404.402578E-521.1087471
TATACAG1800.004340660820.1035692
TCTATGG1850.005086213419.5602282
TAATACT2000.00794681718.1053714
CTATTCT2000.00794681718.1053714
TAAAGGT2050.00916184717.6637744
TATTAGA2050.0091973817.6519132
Produced by FastQC (version 0.11.5)