FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127509
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGT259.4057835E-486.91158
TACACCA553.2245007E-452.6736345
CTAGACT750.001485942938.6273354
CTAACCT800.002040133336.2131234
TAGAGTA1051.8557452E-434.488697
CCTGTAC850.00274673334.082943
GGCGTGA1153.1655966E-431.4896748
ATAGAGT1453.4909703E-529.9694826
TTGTTCC1306.490233E-427.8562494
GTGGGCG1050.00772191327.5909529
CCTAGGA1100.0096885126.3368172
AGGCGTG1100.0096885126.3368177
ATAGAAC1100.0096885126.3368173
GTATAGA1100.0096885126.3368171
GGACAAG1100.0096885126.3368171
CTGGCAC1450.001227842424.974573
GGCACTG1500.001495986124.1420845
TAAATAG1700.003096412221.3018365
TAATGTT2256.8696705E-419.3136655
GTATTTA1950.0068487518.5708311
Produced by FastQC (version 0.11.5)