FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237661
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGC1000.00611796328.9486941
GTTGTAC1100.0097207726.3280753
GTCTATA1400.001007568625.847051
CCTACAC1851.8417221E-423.4817983
CCTTACA1550.001818674323.3555532
ATAGTCA1902.203265E-422.8686716
TATAGTC1600.00218739522.625695
GTATTAG1600.002192755722.6161671
CCTGTAC1650.002609104621.9493058
TATAGAG1650.002615495121.9400632
GCACCAA2104.3339864E-420.6907026
TCTAGGC1750.003679301120.6863463
CTTATAC1750.003679301120.6863463
ACAGGCC2155.0721917E-420.213788
CAGGATC1850.00507522519.5681654
ATAATAC2256.909067E-419.3072573
CTCTTAG1900.00593536819.0451951
GTGTAAG1900.00593536819.0451951
CATAATA2751.2500117E-418.4296532
ACTGAGG2400.001063081118.1081758
Produced by FastQC (version 0.11.5)