FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAATATGCTGACTAATTAAGGCATGGTCTATTTCCAATTCTTTAAA12.1739130434782608No Hit
CCATTCATATTACCCCAGATAAATACATTGAATTGGAATTTACAGATTAC12.1739130434782608No Hit
CGTTTAGGTTTTAGTTTGATCTCAGGTGCACAAATTTCTAACTGCCCCAC12.1739130434782608No Hit
GTGGAAGGCACCATGTCTGCCCATACGGGGAGGATTTGTAATCGCTGAAA12.1739130434782608No Hit
TTCCTCTGCGTCATCTGATGATGAGGAAGTACGTCTAAATTTGCTTGTGC12.1739130434782608No Hit
ATTCCATGCAGTTCCATTAGATGATGATTGCATTCATTTCCATTCGATCA12.1739130434782608No Hit
ATTCAGGCGAGCGCCATTAAAATTTTGTATAAAAGCACCTGTCCAAAGCG12.1739130434782608No Hit
CAGTCCTAGGTCGACACTATTCAAATGGCCTTTTGATAGTTTCAGAAGCC12.1739130434782608No Hit
GAAGGGCACTCGCGTGGAATCGAAGATAATGCAGTGAAGGGGAATCGATT12.1739130434782608No Hit
TCCTATGACTAAAGATGGATAGAGGAATAACATACTAATGTTTCTGTCAA12.1739130434782608No Hit
GTGCCGGGCAATATCACAATAACAGCGTAATAGCCCAAAAAAGTGATTGA12.1739130434782608No Hit
TCATCCTTCTCTTAGACTGGTTTTTATTTATGATCTTTAGCCAACACATA12.1739130434782608No Hit
GCTTAGTTCAGCCCGCTTGAACAATAGAGATGTAGACCAGGATGATGTAA12.1739130434782608No Hit
TTATTATTCTAGGGAAGAAAGTGATCGCTGGGGCTGCGGGGTACGCAGTC12.1739130434782608No Hit
GTTATTACTGACACTCGTAGAAAAAGACGGCACCACAGTACAATCAAAGA12.1739130434782608No Hit
GATATATGTAAAAAGTTCCATGTAGGAACAGCCTAGGCAATAGTTACATC12.1739130434782608No Hit
GGTTCTTATGTTTAAATTACATTTAAATTAGTAAGGCTGTAAATTTTTGT12.1739130434782608No Hit
GTTTCTGTCGGTTGCTTTACCATCCGGCTAAGATATGTACAAANNNANNA12.1739130434782608No Hit
GGTCAGGAGGTTGAGACTCGCCTGGCCAGCAGGGTGAGACCCCCTCTCCA12.1739130434782608No Hit
CTGTATAATAAAACGCAGGGGAAATTTAAATTCAATGGACATGGCTTCAT12.1739130434782608No Hit
CTTTTTTTCTCTGCGATCATTATAAAACTTAATGCTGTGAACAGGACTTA12.1739130434782608No Hit
ATTATCAGCCCACCCATGAAGCAGCCCATTTGAGACCTGAATAGAAAGTT12.1739130434782608No Hit
GTATTAGGAGAGTGTTTTGACTCTGACTTGTGGCATCTATGAAAGATTTA12.1739130434782608No Hit
GAATATTAGGCACTAATAATTATAGTGATCTTGTTTGTTAAAATTGTATG12.1739130434782608No Hit
GATTTCATGATGATACCTCCAAAAGCTAATGCAAAAAAACAAACATTGAC12.1739130434782608No Hit
ATCTTATGAAATGTAAATGTGATAAAGAATGTCAATACAATAATCTAATA12.1739130434782608No Hit
CCATTCCATCCGAATCCATGACATTCTTTTAATTTCTTATCCATTTCAAG12.1739130434782608No Hit
GTGCGGGCGAGGGCCCGTAGTGAGGCGGCGGGCGGCGTTTATGTTCATGT12.1739130434782608No Hit
TAACTGAATAGCAAAATAGAAAGGGCGGAAATTGAGGAATGCTTTCTCAG12.1739130434782608No Hit
CACTCCACTGGGGGCCTGGCGTCCCACAGAGTGGCCAGGCCACGGTCTGA12.1739130434782608No Hit
CATTTCTTTTGTTTGAGCATTTTTGAAACAGTCTTTTTTTAGAATGTGCA12.1739130434782608No Hit
TATATATATACGGATACTGTTTTTTCCCATAAAATCGAGCCCCTGCACGA12.1739130434782608No Hit
GTCTTGGATAAAAGATTTACAATTTAACTGTACTATATATTCAAACATAT12.1739130434782608No Hit
ATCTGCATCCTTGCCAACATCTGTTCTATTCTGACTTTATAACAATGGAC12.1739130434782608No Hit
ATTAAAATGTAAACACATTAAATGAAGTTGTTTTTGATTAACTTGGTGAA12.1739130434782608No Hit
GTCCCAAAACCGGCCCCGCTGTCCCCATGGAACCACCCCACAGCGAAACG12.1739130434782608No Hit
GGATGGAGCAAGATTTGCTAATGCAGGTCTAGCGTTGTCCCCTAAACTAG12.1739130434782608No Hit
TCACCATGCTGATCCAGGTAAGTAGGCGAATCCAGGCTGTACGAAAGAAT12.1739130434782608No Hit
ACTATCTAACCACGTCCTTTACGGAGGTTTTCAGTGTTTGTCTCCTTCTC12.1739130434782608No Hit
TTATCACTAAGCTCATTGACAAAACTAGAGAACCACTGACTATTTTTTTC12.1739130434782608No Hit
GCATGAGCATATCACGTGAGAGTATAGCTCAGTGCAGCCCAGCCCTTAAA12.1739130434782608No Hit
CACTAAAATAGCTTAGGATAGGGATATACTCTGTTTACTGTGTAGTCAAA12.1739130434782608No Hit
ACAATATTAAACTGATTTAAGCCCATTTTGACTCAGTTTCATTTTTGTCA12.1739130434782608No Hit
GAACTTGCAACTGCCTTGGCATTCAGGGTCTCAGAACTGCTGCTACCATT12.1739130434782608No Hit
TTAAAAACAAGTACCCACACACACAAGATGCAAAGGGGGAACCCTGATAN12.1739130434782608No Hit
CGGGCGCCTGCCTGCGGTGGGGGGGGGAAGGGAGGAGGGGGACAAAGCGG12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)