FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300131
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT1000.006086244228.9819584
TAGGGGT1050.007723405527.6018684
GTCTATG2259.1611764E-725.7488631
GTCCATA1952.628719E-422.2826711
GCTCTAG1700.003107976421.2996121
GAGAGCG1850.00506184119.5791427
TGTAACC2256.896559E-419.3148672
GGGTAAG1900.005916725419.0575491
ACCATCC1950.006854229618.5781788
CTAATAC2751.247631E-418.4369183
AGTACCA2400.001062973318.1107065
GATTAAG2400.001065355118.104671
CTGTGTA2851.6359692E-417.795949
ATTGTAC2050.00916223217.6660373
ATATTAC2952.1377439E-417.1840931
TATAGGT2600.001815284316.7203624
GTCTAAT2650.002068703316.3966831
TAAGACT2750.00264023915.8083414
TCTAATA3706.72445E-515.6607022
ACTGTGT3254.4555444E-415.6056728
Produced by FastQC (version 0.11.5)