FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n02_S_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n02_S_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287242
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTG1855.8520254E-627.3992525
GGATAGT1550.001821808723.3506771
TTAGACA3203.4076947E-822.6288474
TAGCACC1650.002614694621.9431274
ACTGTCC2003.1136733E-421.7312628
CTAAGAT3055.375059E-721.3675673
GTCTAAG2053.696365E-421.1864681
TAAGACT2455.1454655E-520.6892324
TCTATAC2155.085397E-420.2080883
CATAGAC1800.00432994720.114533
TATACCC1800.00432994720.114535
TATATAC4001.6421836E-819.9133853
GTAAGAC1900.005919122619.0558723
ATACTGC2307.998589E-418.893466
ACTCTAG2308.026748E-418.8835931
GGCTAAG2308.026748E-418.8835931
TACTATG1950.00686991618.5704947
GTCTTAT2359.2749175E-418.4818131
CTATATA2751.24983E-418.4322244
TAGACAG3953.143159E-718.3322325
Produced by FastQC (version 0.11.5)