FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380289208
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43336071.139555608951175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG3003950.013.7804575
GTATATA5567950.013.7350951
GTATTAG2397650.013.1866131
GTATAGA2286050.012.4416121
GTATAGG1408050.012.4008681
GTGTATA3736350.012.1458951
TCTATAC2188700.012.09678653
TATACAG3099650.011.7747415
GTATTAT3791200.011.6967761
CCTATAC1385400.011.6841123
CTATACT2235550.011.4121654
TACACTG2924650.011.2727425
TATATAC5586700.011.0917493
GTCCTAG1569500.010.912791
CTAATAC2343450.010.6981363
TAATACT3325900.010.6228484
ACTGATC2589100.010.5966298
ATAATAC3774150.010.3537823
GTGTAGG1702500.010.2816641
ATATACT4295500.010.0079944
Produced by FastQC (version 0.11.5)