FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_negctrl1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_negctrl1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATGTCACAATGCCCCCTTAGTCAGAGCCTAAACAAAAACCCCATC10.6944444444444444No Hit
GTAGTGGTGATATCTCGGATCACTGCCACTTATGCCAACATTCTCAAGCG10.6944444444444444No Hit
CAAACAATCTGATCATAATCCAAATACAGAGGTTTAAATTCTGTAAAGCT10.6944444444444444No Hit
ATATATAATAAGGTGTAAAAGACTAAAAGCAGGACTTACATGGTACTAAT10.6944444444444444No Hit
GGATCTGGCGCCGCATGAGGGCTCCGACCAGGGCGGACGTGGCCGGGAGG10.6944444444444444No Hit
GTCCATGATTGCGCATAAATCGGCTCTTGTCCACATAGCCGTTGATGCAG10.6944444444444444No Hit
GGCGCAGTCACATCTTACATGGCCAGAGCAGAAGGAAGAGAGAGAGCGGG10.6944444444444444No Hit
GTCCCGTAAAAGCTACCATGATCTACGAGATTAAGCTAGCTAAAGTCTTA10.6944444444444444No Hit
GTTTGTGTGATTTTTACGTTTCAAGTTCTTTCCATCCTCACTCTCCTTGC10.6944444444444444No Hit
CTGGTTGACACTCTCTCTATTTCACTTGTTAGTACCTCAAGAAAATTGTT10.6944444444444444No Hit
TTCATTGGAGACGGCATGTGGGACGTGCTGCAAGGCTTGAATGCGCTCCG10.6944444444444444No Hit
GTGGGCGATCAGCAGGACTTGTTTTCTGAGCACTTGTTCCAACCCTGCTG10.6944444444444444No Hit
CTCATGACCTCAGGTGATCTGCCCCCCTTGGCCAGCCCAAATTGTTGGGA10.6944444444444444No Hit
ATTGCTAGCTATGTGCCCTCTTGCTTGAGGTCACATAGCTAGCAATGGCT10.6944444444444444No Hit
GATGGGGCTGATGGGCTTGGTCTTCTCATATGGTGTCATGGACCTTTCAG10.6944444444444444No Hit
GGGGGGTGGGGAGGATGGTGGTGATGGGGCTGATTGTGATGATGATGGTG10.6944444444444444No Hit
AGCTGATCCTGGAAGGAGGGTAGCTGAAGAATGACCTCCTCAAACACAAG10.6944444444444444No Hit
CCCCAGCAATGGCTATTAACCAGAATGAAATGACAGACACAGAAATGAGA10.6944444444444444No Hit
GACTTAGGAAGATCCAAATTGTTACTGATGAAAGCTACAGATTTATAGTT10.6944444444444444No Hit
CTCTCCATGCCTCACTTTTTCCACTCATCATAGGAAAAGTATTCCTGAAA10.6944444444444444No Hit
GTGGTAGCATGTAGTCCACAAGCCGGCGTCCTCCCGCCATCCAACCGAGC10.6944444444444444No Hit
GAGTTTTTCTGCCTGATAGGTAACGCCCACATTGCTTTTATCCAACAATA10.6944444444444444No Hit
CACCAAATAAATAATAGATGAAAGTAGGTTGTTATTCATGCTAAAAAAAA10.6944444444444444No Hit
GAGTGTACATGATTATATTAAAATAGATAGGAATAGTTACTATGATAAAG10.6944444444444444No Hit
GTTGGGCCCCGCCTGAATTCTCCCCGGGACCTGGGGTTGTAGTGTGCCCT10.6944444444444444No Hit
ATTCCATTCCATTCCACGCGGGTTGGTGCCATTCCAGTCCTTTCACCTTC10.6944444444444444No Hit
CCGCATGGATTGATGACTACACTAATGGCTTTCTACTCTTCTTTCTCACT10.6944444444444444No Hit
GTCTGTGTTCCACGCATTACAAAATATGGAGGACTCGGGAGTTATAGAGG10.6944444444444444No Hit
GTGATTCTGCTGTCTCGACTTAAAAGTATGTTTTAAATTTTATAATAATT10.6944444444444444No Hit
GGCCCCAAATATTGGCACGAGAATTAAGTTTATGTGGCGGAATCGTATGG10.6944444444444444No Hit
TTTTTAAGGCTGACTTCCTCCTCATAACATATTTGTGAGAGTCATCTATG10.6944444444444444No Hit
AGGATGTTTCTTTCAACTTTAAAATTCTATGATCTTATCTTCCTGTCATG10.6944444444444444No Hit
CATCACCTGCGTGCACGACATTGAAGGCGCCCAGGAATGGATAGGCGCCT10.6944444444444444No Hit
GGTCCCTGTAGGAGCCTCTGCAGTAGCGGAAGGTCTGTCTTCGTTAGTCG10.6944444444444444No Hit
GGCTGTTCCTTGGTCGCCACCGCTAGATTGCTCACTCATATCTTTGTCAT10.6944444444444444No Hit
CCCAGTGAGTGTGCTGTTTATGCTTTTCCGCATCCTCTGCTGCGTTTGCT10.6944444444444444No Hit
GAACGAGGGGACATAGCAAGGGAGAAATCCCACCACTTATTAAAGCAGCA10.6944444444444444No Hit
ACTCCATCCCACTTTCCCATTCAACAGCAAACTCAAGATTCCACTGAATC10.6944444444444444No Hit
GATATATAATATGTATTATCCACATTCTAGAGCTGAGAATATTTTTAGAT10.6944444444444444No Hit
GCCGAAACGAGATTTTAGGCGCTCTTCAATTTTTTCAATTTCACGCGGAT10.6944444444444444No Hit
CTTCCTTGGCTTGGCAAAGAGGCAAAGCACTCTTCTCCCCTCCCTCATAA10.6944444444444444No Hit
CTGAGGGGAGGAGCCGCCCTGATGTTCGCATCACGCGAGACCTACTCAAA10.6944444444444444No Hit
ATAATCGCAAACATGACCCAGCCACAGCAATAAAGCCGATCCACATCAAG10.6944444444444444No Hit
GGCAAATGCCTGTCTATAAAAATAGAGAAAGATAAAGCAGATGGTCTAAA10.6944444444444444No Hit
TGCCTGTAAATCCCAGCACTGTGGGAGGCTGAGGCAGACGGATCGCTTGA10.6944444444444444No Hit
ATAATAAGATATACCTCTTAGATACCAGTCCCTTTTTTTTAATGAGGCCA10.6944444444444444No Hit
CGGCAAAATCGCGCCATTGAATTTGGCGGAACGAGCCATCCGCAGTTTGC10.6944444444444444No Hit
GAGATGTTCAGAGGATCCCAGGTGGGTGGTATGTGAGTGGTGGTAGCAGC10.6944444444444444No Hit
CTTCTAAAAGACATAAATTTCTTGCAAGCATAATTTCAGATGGATGGATA10.6944444444444444No Hit
AAAATATACACTCTCCATAAAAAAGATAACACAACAACAGATCAAACACA10.6944444444444444No Hit
GGTCAGTATTTTGCCGCTCATCGCCGTAGCGCTTTGCCGTATTGACGCCG10.6944444444444444No Hit
GCGTTACAATGCCTACGGTTGGCATACAGCCTTGGTAGAAGATGGAAGAG10.6944444444444444No Hit
GGGTGGATCACTTGAGGTCAGGAGTTCGAGACCAGCATGGCCAACATGGT10.6944444444444444No Hit
GTATATACCACATTCTGTATATCCATATTTATCCATCAAGACACATTTGG10.6944444444444444No Hit
CACGGGGCTCCCCTCCAGCTCGCTGCTTTCCCAGGTGGGGCCGAAGTATC10.6944444444444444No Hit
GTATTAAAAAAATTAAGACAGTGTCTCACTATGTTGCCCAAGCTGGTCTC10.6944444444444444No Hit
GGGGGGGGGGGCGGGCCGGCCTATGGCGTTGAGGAGGGAGGATTTACCGG10.6944444444444444No Hit
GTGCATTTCGAAGGAACCATGAATTGTATCCAGAGCCTCGCCCAAAGTTA10.6944444444444444No Hit
GGGTGGATATCAATTCCGTAGACACATGGATAGGCAAGCGCAGGACTCCC10.6944444444444444No Hit
CGGCATGGGTCTTACCTAACCTTAATCTGCAGACTAACTCCTAATAAACC10.6944444444444444No Hit
GCTGCCTACTGACTATAGCCTTCAGAGCACTGTGGCCTATAAAGATTTTC10.6944444444444444No Hit
GGTGAGTGTACTCAATACTTAACTGTATGGTACCATCTTAGAACATCCCA10.6944444444444444No Hit
CACCACCAAGGTGCGTATCACCATTGGTTGCTTTAACCTCACACACGCCG10.6944444444444444No Hit
CAACCAGATAGGCGCAAGCAGGCAGACAAAAAACAGTGCGGGGTGCTGAC10.6944444444444444No Hit
CGCCACTCTATCCTTGCTGATGAAGCATTGGTTAGAGCAGCTGCCAGAAT10.6944444444444444No Hit
GCCGGGGACTTCCCTAGGCTGGACCCTATAGTAAACAGATATCAGGCACA10.6944444444444444No Hit
GGGGGGGGGGGGTCCCGGCCAGTGCGCCGCGGCCTGGCGCCCGCTGACTG10.6944444444444444No Hit
GCGAAGAACATAGCCGCAGAAGGGGCTGTTCTATATGGTAAAGTTGATGC10.6944444444444444No Hit
GCTCGTTCGTCAGCCTCCGGAGGAGCTAGACTTAACGGATTGTGCCACCA10.6944444444444444No Hit
GGCGGGTGACTTCAAGGGAGAAACAATCCTGGTACTCTCCTGGGACAAAA10.6944444444444444No Hit
AGTTTGCAGTGAGCTATGATCATGCCACTGCACTCCAGGCTGGGCAACAG10.6944444444444444No Hit
GTACCGTAGTAAATATCATACTCGACATCATATTTGTGGGACCACTAGGG10.6944444444444444No Hit
GTGCTAGGCTCCTTTAAACAGACAGCTACTGTGTGTAATAAGTGAGGACT10.6944444444444444No Hit
GAGGGACGGTGCTCCACATGATAGCGAAAAGGTTCCACAACTAATTTCAC10.6944444444444444No Hit
CCTATGTCCTGAATGGTATTGCCTAGGTTTTAATAAAGGTTTTTTAGGGT10.6944444444444444No Hit
GGGGATAGCATTGGCCATAGAAACATCGTGATAGGACCCTAAGAGAAGGC10.6944444444444444No Hit
AAAATTCAATATTATAGATTTAAAGGGGTTAAATATTAACTTTGAGAATC10.6944444444444444No Hit
GCCATCCCCTTGGCATTTGAGGGTGAAGCAGGGTGGCTGAACATAGCAGG10.6944444444444444No Hit
GTGTTAAAAGTGACAGCAAAACAAAATTAAAACTACTGTAGTAATAGTGC10.6944444444444444No Hit
GCTGTTCCCAAAAGGAAGGTAGAGGCACTGGAGGACCTGAGTCATGGTGG10.6944444444444444No Hit
CTCCTGTACGCGCGCCGCCTCCGTCTGGGTGCGCGCCAGCAGGGTCCGTG10.6944444444444444No Hit
GTGGTGGACTGAGTGCTTGCCTCCCCTCTGCAGAGGAGTATAAGCTTATT10.6944444444444444No Hit
ATTTTATTTGTTAAGCCAATACAACGACATTCTTGACGCATATAAACGAC10.6944444444444444No Hit
CTTCACGAGAGTGTAATAACTCCATGAGAGGTTCTAAGCGAGCTGCTATT10.6944444444444444No Hit
GGCGGGGACCCATGATGTTGTCAAATTCTAGTTGTAAAGCTTTCCTTTTT10.6944444444444444No Hit
GGGTGAATTATGTCATCATCGGAGTTAATGTACATTGTGACTCCAGATCA10.6944444444444444No Hit
CGTGCGGTCGTGGCCAGTGGCGGCCGGGCTTCAGTGTGGGGGGAGCGGAG10.6944444444444444No Hit
GCCCAGCACGATGTGAGGAAAAGGCGGGTTGCTCCCTTAAGGTCACGAAT10.6944444444444444No Hit
GTGTAAAGGGAAAAAAGAGAGAGAGATAATGAAGAAATCAGCATGCATGA10.6944444444444444No Hit
GTAGTGTGAAAGGCCTAAAGTGCATTTCCATTTTTTGGCCCTCTTGAGTC10.6944444444444444No Hit
ATCTCAAATAGGGTCTTCTCATTCACCCTGATAAGGCACTGAAGACGCCA10.6944444444444444No Hit
GTATTATGTCATCTAACAAAATAAATAATACTGTAATATCATCTAATCCC10.6944444444444444No Hit
GTTTCTAACTAAATTGCCAGAAGATATCACATCGGAAAAGCTGTTCAGTT10.6944444444444444No Hit
CTCATCTAAAGAAGTATTAGAATCAACTCAGGTTTTATTGGTGAGTTCAG10.6944444444444444No Hit
GGCTATAGTCAATACAGATCAGCCACCCTAATGTATATGTAGTGAAATTA10.6944444444444444No Hit
ATAGAGAGGTGAAGCAAGATGGCAGAATCAAGGGTTCCATCCATTGTTCC10.6944444444444444No Hit
GGCGAAAGTATAAACTATATTACTGCTGAATGTTTGAAAAAGCCATGTTA10.6944444444444444No Hit
CTTTAATCCAAGACGTTTAAAGAATCCCTCGTCCAGTAATTCTAGTGAAT10.6944444444444444No Hit
CACTAGATGATGCCTGTGTTCCCCTCTACTTGTCTGTTAGCCCCAGCCAC10.6944444444444444No Hit
TGGGGTGGCTACTAATTCTCTATTTTTCACCTTTCCTTTGTCATAATTTT10.6944444444444444No Hit
GGGGCGGGGGGAGTGATGGGGTGCATGCAAGCAAGTTGGAAACCACTGTG10.6944444444444444No Hit
GTGCTGTCACCAGGCTGTAGTGCAGTGGAACTATATGTCGCACTGCAACC10.6944444444444444No Hit
TCTCTGTCCCCAATCAAATGTCATCTTCAATTCACACATGTTGTGAGAAG10.6944444444444444No Hit
GGGGGGGGTGTCCCAGCTGTTGGCGTTTAGGATACATCCGCAATTGGGCT10.6944444444444444No Hit
GGGGGGGGGCCGCCTGTGGGAGACTACAGGAGGGCTCACAAAGACGGCCG10.6944444444444444No Hit
GGTATTTATAATGTTTCTACCAATACGGGAACATCTGTAAATGAGGTGAT10.6944444444444444No Hit
ACTTCACTGCATGTGTGGGCTTCGGTTTTTTTTTTTTCCTTCAAAAAAAA10.6944444444444444No Hit
AAAATGAACAGGTGAAGAGCTGGAGCAATAGGATATCAATGACAACAAGT10.6944444444444444No Hit
TGGCAGAACATTGGGCTTCGGAGGGTGCGAACAAGGTAAAAAAAAAAAGA10.6944444444444444No Hit
GGCGAGTCTTGAAGAAATAGGAGAAAAGAGTAGAAGAAAGCTGGGGAGTG10.6944444444444444No Hit
GCGGTGCATGCTACAGCGAAATCGTGTTGCAAGGACGAGCGAAGCAATGT10.6944444444444444No Hit
GGAAATATCAACGCTTGGCGTACATTAGCTGACGATATGTATCCTCCTCG10.6944444444444444No Hit
AGCCAGAGCCACAGGCGCCTGACCTGTAAATCACTGCAGTCCCAAGTTCA10.6944444444444444No Hit
CAAGATGGATCAAAGACTTAAATGTAAGACCTAGGAGCATAAAAATCCTA10.6944444444444444No Hit
GTATTAAAGGCTTATTGAAAAATGCTAGTCCTGCCAACCCTCTCTTGTAC10.6944444444444444No Hit
GGGGGGGGGTGGGTCCCCGGGTGGCGCACGGCAATCGACTCCTCCCAGGT10.6944444444444444No Hit
GGGTTAGAATATATTAGCAATATTTCAAAAGACTTTGTTTTGAAATTACT10.6944444444444444No Hit
TACGGAAAGAGACCAAACCTTTAGGAACGCTACCAGACGCTTCGTAACCG10.6944444444444444No Hit
GGGTGAACAGAACAAGCCTTCATATCAAGAAAAGAAAAAAGAAAAAAAAG10.6944444444444444No Hit
GGGGGGTGGCACACCCCTGTAGTTCCAGCTACTCAGGAGGCGGGGGCGGG10.6944444444444444No Hit
CGTGGATAATGGTGAAAGTATCGATACGAATATAATTCATTTCTTATGCT10.6944444444444444No Hit
ACGCCTGTAATCCCAGCTCTCTGGGAGGCTGAGGCAGGTGGATCACCTCA10.6944444444444444No Hit
ATTAAAAAAACAAGGAAATATTTATCTCCCATCTCACTCTCAGTATTATC10.6944444444444444No Hit
TCCTAACCCCATATACTCATTATAGCTCGTCTCTTCACCCCGCCCATCAT10.6944444444444444No Hit
GTGGGGGGCAGTGGCAGCCTCTGCGCGCCCGCCACCCTCAGCCTCCCGTG10.6944444444444444No Hit
GATTCAAAATATTAATGATTGAACTCTAGTTCCAACCCAACAACCAACAT10.6944444444444444No Hit
TCGGAAGAGTATCTTTCCAGTATTTTAATAACAGTGTCAACCATAGATCT10.6944444444444444No Hit
GGGTGCGTGGACACAAGCAGCCGTTGTGACGCGCTCTCAAGCACCTCTAA10.6944444444444444No Hit
TTGCAAGTCATATGAAGTTAGCCTCAGACCACCTGCCTCAAATTACGTTA10.6944444444444444No Hit
CATTAAGGGTGTGATCGGCTGATACGCTAGATTAACTCGAGTACCTTGAA10.6944444444444444No Hit
GGGGGGGGGGGGGCGGTCCACCCCCCCCCCTGGGGGCTCGGCGACCGGAG10.6944444444444444No Hit
GCCCAGATTGCGCCACTACCCTCCAGACCAAGACTCCTTCGCAAAACAAA10.6944444444444444No Hit
GGTATAAAATATCTTAGAGGCTAGGGACAAGGAGATATCATTTTAAAAAT10.6944444444444444No Hit
TTTCACTGCTATTCTCTCGGGTTCAGTGATGTTAACTGGTGCTTTGAGTA10.6944444444444444No Hit
GGGTGATGCGGAGCAATGCTGCCCACATTTCCCTCGGTCTATCAACGGTT10.6944444444444444No Hit
GTAGGCCAAATGAAAATAAAATAAGTTGTTTTCTCTCTGTCGTGTGCGAC10.6944444444444444No Hit
AGTCACCACAATGCGGAAGAACGGCAAGGCAAACAGGAAGAAACCGTACT10.6944444444444444No Hit
GGCGGGCCTTGCTGGGCTTCCCAACTCGGTGTACGATTTCGGGAGCTCGA10.6944444444444444No Hit
GGGATTATAGCTTCAGTAAAAAAGTTAAAAACTGAAAAGTGCTATGCAAA10.6944444444444444No Hit
GGGGAGAGAGGCTGCTCCTGATATTGATAAGTAGAGTGGAGGCTATGTAA10.6944444444444444No Hit
GTCACATTTTCACGGCTTTCGGCCATAAACTGAGTGACATTAGAAAGTTT10.6944444444444444No Hit
ACCAAGATCAGAGCAGAACTAAGTGAAATTGGAACAAACAGAAAATACAA10.6944444444444444No Hit
GGGGGGGTGCTGAGTCTTTCTGTTTAGTGGGAACTTTGCCTAGATCCGCG10.6944444444444444No Hit
ATATATATCACATATAGGTGATATATAAATATATTGATATAAATATAAGA10.6944444444444444No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers