FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_negctrl1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_negctrl1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180742
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2390.13223268526407808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAG700.001051542541.4509541
GCCTAGC1050.007671762327.633971
TAACCCA1100.00963864526.370585
CATAGAG1751.2479333E-424.863692
GTATATA4151.8189894E-1224.4710461
CTACACA1851.8193452E-423.5197074
TTTGTAC2003.0839484E-421.7557283
GTGTTAA1800.004280240720.1497731
TTAATGG1900.005860709619.0839734
ACCTGGG1900.005870092719.0786917
GTGCATA1950.006798166318.5997891
TACACAG2751.2325752E-418.4594085
TATATAC4755.7425495E-918.3206163
GGTGTTT2050.00908452617.6875866
ACACTGT2600.001800816916.7351786
ACACAGT2750.00261927215.822356
CACACAG3050.005216137514.2660525
ATACCAT3050.005216137514.2660526
ATATGTG3350.00970446112.9884955
ATTATAT4856.5760943E-411.9619133
Produced by FastQC (version 0.11.5)