FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_81_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_81_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178752
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT850.002732267434.127131
GCGAGAC900.003620359632.2221643
CCTGTAC1050.007691877427.6189943
TATAGTG1050.007691877427.6189945
GTCCATG1606.7844754E-527.1950531
TCCTGTA1409.962535E-425.8928072
CCCCAGA2001.0591157E-525.374952
TACAGTG1851.822515E-423.5134687
ACACCTT1550.001802731323.3870536
GTATATA2202.2108188E-523.0745931
ATATTGT1952.6031505E-422.3076486
GGGGCGG2505.926733E-520.305641
CCCACAC2155.033904E-420.232523
ATACAGT1800.004293524720.1388516
GGCCAGA1850.005022904419.6000391
AGGTGTG2307.9271523E-418.9130067
GCCCCAG2359.142746E-418.5157811
GGGGGGG6750.018.2643341
ATATATG2450.001211640917.7550683
GTGTGAG2050.00909805417.6828949
Produced by FastQC (version 0.11.5)