FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_72_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_72_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187752
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA259.375337E-486.9987261
GGGGTAG700.001054523641.4279631
CTACACC750.001480422838.66614
CTATACT800.002032558736.2494664
GTATATA3200.031.7182831
GATGGTC1050.00769332627.6186437
TATATAC2901.1777956E-824.9996323
GGGCAAG2151.8555025E-523.6043031
ATAAGGT1902.184183E-422.89443
GGGGATG1700.003083820221.3232151
TACAGAG1700.003083820221.3232157
TACACAG1700.003083820221.3232155
TAAGGTT1750.003648139520.7139824
GTGCTGC2155.035636E-420.232266
ATATACT2205.879479E-419.7724364
CTAAAAC1850.005032340519.5943073
CCATGAG2256.839953E-419.3330482
ATACAGA2400.001055459618.1247356
CTTTAGA2000.00789344718.1247332
TACATGA2050.00910038417.6826674