FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_54_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_54_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG450.00961386948.3572241
GGTAGAC1204.051475E-430.2082313
CTACCCT2156.100945E-726.976654
GGGGTAC1100.00963783426.3766691
GCACGGT1100.00966124226.3635463
GTGCTAG1701.0255036E-425.6008821
GGGTACA1500.001487570524.1786121
TATAGTC1801.5168883E-424.1665845
GTTATAC1550.001805610723.3870163
TACCCTG2502.2705171E-623.199925
TAATAGT2953.8559665E-722.1185674
GGGTGTA2303.1207135E-522.0761221
GGGGTAG2053.6460534E-421.231
CTAACCA1750.003652963320.7142169
TATAGTG2205.89039E-419.7726615
TAAGAGT2256.8526185E-419.3332674
GGGTAGA2307.9179736E-418.922391
TATACTG2701.07602405E-418.7962325
GAGTATT1950.006829881618.589687
GTGAGAC3151.6772345E-518.4126363
Produced by FastQC (version 0.11.5)